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首页> 外文期刊>Applied Microbiology >Role of Mrx Fimbriae of Xenorhabdus nematophila in Competitive Colonization of the Nematode Host
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Role of Mrx Fimbriae of Xenorhabdus nematophila in Competitive Colonization of the Nematode Host

机译：Xenorhabdus nematophila的Mrx菌毛在线虫宿主竞争性定居中的作用

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Xenorhabdus nematophila engages in mutualistic associations with the infective juvenile (IJ) stage of specific entomopathogenic nematodes. Mannose-resistant (Mrx) chaperone-usher-type fimbriae are produced when the bacteria are grown on nutrient broth agar (NB agar). The role of Mrx fimbriae in the colonization of the nematode host has remained unresolved. We show that X. nematophila grown on LB agar produced flagella rather than fimbriae. IJs propagated on X. nematophila grown on LB agar were colonized to the same extent as those propagated on NB agar. Further, progeny IJs were normally colonized by mrx mutant strains that lacked fimbriae both when bacteria were grown on NB agar and when coinjected into the insect host with aposymbiotic nematodes. The mrx strains were not competitively defective for colonization when grown in the presence of wild-type cells on NB agar. In addition, a phenotypic variant strain that lacked fimbriae colonized as well as the wild-type strain. In contrast, the mrx strains displayed a competitive colonization defect in vivo . IJ progeny obtained from insects injected with comixtures of nematodes carrying either the wild-type or the mrx strain were colonized almost exclusively with the wild-type strain. Likewise, when insects were coinjected with aposymbiotic IJs together with a comixture of the wild-type and mrx strains, the resulting IJ progeny were predominantly colonized with the wild-type strain. These results revealed that Mrx fimbriae confer a competitive advantage during colonization in vivo and provide new insights into the role of chaperone-usher fimbriae in the life cycle of X. nematophila .
机译：线虫Xenorhabdus nematophila与特定昆虫病原线虫的传染性少年（IJ）阶段相互关联。当细菌在营养肉汤琼脂（NB琼脂）上生长时，会产生耐甘露糖（Mrx）伴侣-蘑菇型菌毛。 Mrx菌毛在线虫宿主定殖中的作用仍未解决。我们显示在LB琼脂上生长的线虫X.产生鞭毛而不是菌毛。在LB琼脂上生长的线虫X.nematophila上繁殖的IJs的定殖程度与在NB琼脂上繁殖的IJ相同。此外，当细菌在NB琼脂上生长以及与共生双生线虫共注射到昆虫宿主中时，后代IJ通常被缺乏菌毛的mrx突变菌株定殖。当在NB琼脂上存在野生型细胞时，mrx菌株在竞争方面没有定植缺陷。另外，缺乏菌毛的表型变异菌株和野生型菌株也被定殖。相反，mrx菌株在体内表现出竞争性定植缺陷。从注射了携带有野生型或mrx菌株的线虫混合物的昆虫获得的IJ后代几乎只被野生型菌株定殖。同样，当昆虫与非共生型IJ以及野生型和mrx菌株的混合物共同注射时，所得的IJ后代主要被野生型菌株定殖。这些结果表明，Mrrx菌毛在体内定殖过程中具有竞争优势，并为伴侣-菌毛菌丝在线虫X.nematophila生命周期中的作用提供了新见解。

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