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首页> 外文期刊>Applied Microbiology >Versatility in Corrinoid Salvaging and Remodeling Pathways Supports Corrinoid-Dependent Metabolism in Dehalococcoides mccartyi
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Versatility in Corrinoid Salvaging and Remodeling Pathways Supports Corrinoid-Dependent Metabolism in Dehalococcoides mccartyi

机译：Corrinoid打捞和重塑途径中的多功能性支持Dehalococcoides mccartyi中Corrinoid依赖的代谢。

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Corrinoids are cobalt-containing molecules that function as enzyme cofactors in a wide variety of organisms but are produced solely by a subset of prokaryotes. Specific corrinoids are identified by the structure of their axial ligands. The lower axial ligand of a corrinoid can be a benzimidazole, purine, or phenolic compound. Though it is known that many organisms obtain corrinoids from the environment, the variety of corrinoids that can serve as cofactors for any one organism is largely unstudied. Here, we examine the range of corrinoids that function as cofactors for corrinoid-dependent metabolism in Dehalococcoides mccartyi strain 195. Dehalococcoides bacteria play an important role in the bioremediation of chlorinated solvents in the environment because of their unique ability to convert the common groundwater contaminants perchloroethene and trichloroethene to the innocuous end product ethene. All isolated D. mccartyi strains require exogenous corrinoids such as vitamin B_(12) for growth. However, like many other corrinoid-dependent bacteria, none of the well-characterized D. mccartyi strains has been shown to be capable of synthesizing corrinoids de novo . In this study, we investigate the ability of D. mccartyi strain 195 to use specific corrinoids, as well as its ability to modify imported corrinoids to a functional form. We show that strain 195 can use only specific corrinoids containing benzimidazole lower ligands but is capable of remodeling other corrinoids by lower ligand replacement when provided a functional benzimidazole base. This study of corrinoid utilization and modification by D. mccartyi provides insight into the array of strategies that microorganisms employ in acquiring essential nutrients from the environment.
机译：类胡萝卜素是含钴分子，在多种生物中起酶辅因子的作用，但仅由原核生物的一部分产生。特定的类胡萝卜素通过其轴向配体的结构来识别。类皮质激素的下轴向配体可以是苯并咪唑，嘌呤或酚类化合物。尽管已知许多生物都从环境中获得了类胡萝卜素，但对于任何一种生物可以作为辅因子的类胡萝卜素的种类仍未得到广泛研究。在这里，我们研究了在Dehalococcoides mccartyi菌株195中作为类固醇依赖代谢的辅助因子的类固醇的范围。由于在独特的能力下，转化环境中常见的地下水污染物全氯乙烯的能力，脱卤菌细菌在氯溶剂的生物修复中起着重要作用。和三氯乙烯制得无毒的最终产物乙烯。所有分离的麦卡氏梭菌菌株都需要外源性类固醇，例如维生素B_（12）才能生长。但是，像许多其他依赖类海百合的细菌一样，没有一个特征明确的麦卡氏梭菌菌株能够从头合成类胡萝卜素。在这项研究中，我们调查了D. mccartyi菌株195使用特定类固醇的能力，以及将进口类固醇修饰为功能形式的能力。我们表明，菌株195只能使用含有苯并咪唑低级配体的特定类胡萝卜素，但当提供功能性苯并咪唑碱基时，能够通过降低配体的替代来重塑其他类胡萝卜素。麦卡迪氏菌对类胡萝卜素的利用和修饰的研究为微生物从环境中获取必需营养素所采用的一系列策略提供了见识。

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