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首页> 外文期刊>Applied Microbiology >Role of Bacterial Communities in the Natural Suppression of Rhizoctonia solani Bare Patch Disease of Wheat (Triticum aestivum L.)
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Role of Bacterial Communities in the Natural Suppression of Rhizoctonia solani Bare Patch Disease of Wheat (Triticum aestivum L.)

机译：细菌群落在自然抑制小麦茄枯萎病中的作用

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摘要

Rhizoctonia bare patch and root rot disease of wheat, caused by Rhizoctonia solani AG-8, develops as distinct patches of stunted plants and limits the yield of direct-seeded (no-till) wheat in the Pacific Northwest of the United States. At the site of a long-term cropping systems study near Ritzville, WA, a decline in Rhizoctonia patch disease was observed over an 11-year period. Bacterial communities from bulk and rhizosphere soil of plants from inside the patches, outside the patches, and recovered patches were analyzed by using pyrosequencing with primers designed for 16S rRNA. Taxa in the class Acidobacteria and the genus Gemmatimonas were found at higher frequencies in the rhizosphere of healthy plants outside the patches than in that of diseased plants from inside the patches. Dyella and Acidobacteria subgroup Gp7 were found at higher frequencies in recovered patches. Chitinophaga , Pedobacter , Oxalobacteriaceae ( Duganella and Massilia ), and Chyseobacterium were found at higher frequencies in the rhizosphere of diseased plants from inside the patches. For selected taxa, trends were validated by quantitative PCR (qPCR), and observed shifts of frequencies in the rhizosphere over time were duplicated in cycling experiments in the greenhouse that involved successive plantings of wheat in Rhizoctonia -inoculated soil. Chryseobacterium soldanellicola was isolated from the rhizosphere inside the patches and exhibited significant antagonism against R. solani AG-8 in vitro and in greenhouse tests. In conclusion, we identified novel bacterial taxa that respond to conditions affecting bare patch disease symptoms and that may be involved in suppression of Rhizoctonia root rot and bare batch disease.
机译：solani AG-8引起的小麦纹枯病裸露斑块和根腐病，发展为不同的矮化植物斑块，限制了美国西北太平洋地区直接播种（免耕）小麦的产量。在华盛顿州里兹维尔附近的一项长期种植系统研究的地点，在过去的11年中，观察到了根瘤菌斑病的减少。使用焦磷酸测序和针对16S rRNA设计的引物，分析了植物内部，斑块外部，斑块外部和回收的斑块中植物的块根和根际土壤中的细菌群落。在贴片外的健康植物的根际中，发现酸性菌属和芽孢杆菌属的类群的频率高于贴片内的病态植物。在回收的斑块中发现了较高的频率发现了虫和酸性细菌亚组Gp7。在患病植物的根际中，从斑块内部以较高的频率发现了几丁质，Pedobacter，草酸杆菌科（Duganella和Massilia）和拟杆菌。对于选定的分类单元，趋势通过定量PCR（qPCR）进行了验证，并且在温室中进行的循环实验中重复了观察到的根际频率随时间变化的情况，该实验涉及在已接种根瘤菌的土壤中连续种植小麦。从贴片内的根际中分离出可售细菌，并在体外和温室试验中显示出对solani sol。AG-8的显着拮抗作用。总之，我们确定了新型细菌类群，其对影响裸露斑块病症状的条件有反应，并且可能与抑制根瘤菌根腐病和裸露斑病有关。

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