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首页> 外文期刊>Applied Microbiology >Biotransformation of Trichoderma spp. and Their Tolerance to Aromatic Amines, a Major Class of Pollutants
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Biotransformation of Trichoderma spp. and Their Tolerance to Aromatic Amines, a Major Class of Pollutants

机译：木霉属物种的生物转化。及其对主要污染物芳香胺的耐受性

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Trichoderma spp. are cosmopolitan soil fungi that are highly resistant to many toxic compounds. Here, we show that Trichoderma virens and T. reesei are tolerant to aromatic amines (AA), a major class of pollutants including the highly toxic pesticide residue 3,4-dichloroaniline (3,4-DCA). In a previous study, we provided proof-of-concept remediation experiments in which another soil fungus, Podospora anserina , detoxifies 3,4-DCA through its arylamine N -acetyltransferase (NAT), a xenobiotic-metabolizing enzyme that enables acetyl coenzyme A-dependent detoxification of AA. To assess whether the N -acetylation pathway enables AA tolerance in Trichoderma spp., we cloned and characterized NATs from T. virens and T. reesei . We characterized recombinant enzymes by determining their catalytic efficiencies toward several toxic AA. Through a complementary approach, we also demonstrate that both Trichoderma species efficiently metabolize 3,4-DCA. Finally, we provide evidence that NAT-independent transformation is solely (in T. virens ) or mainly (in T. reesei ) responsible for the observed removal of 3,4-DCA. We conclude that T. virens and, to a lesser extent, T. reesei likely utilize another, unidentified, metabolic pathway for the detoxification of AA aside from acetylation. This is the first molecular and functional characterization of AA biotransformation in Trichoderma spp. Given the potential of Trichoderma for cleanup of contaminated soils, these results reveal new possibilities in the fungal remediation of AA-contaminated soil.
机译：木霉属。是对许多有毒化合物具有高度抵抗力的大都会土壤真菌。在这里，我们显示木霉和里氏木霉对芳香胺（AA）具有耐受性，芳香胺是一类主要污染物，包括高毒性农药残留3,4-二氯苯胺（3,4-DCA）。在先前的研究中，我们提供了概念验证性的补救实验，其中另一种土壤真菌Podospora anserina通过其芳基胺N-乙酰基转移酶（NAT）（一种使乙酰辅酶A-依赖AA的排毒。为了评估N-乙酰化途径是否能使木霉属（Trichoderma spp。）中的AA耐受，我们克隆并鉴定了特里氏木霉和里氏木霉的NAT。我们通过确定重组酶对几种有毒AA的催化效率来表征重组酶。通过补充方法，我们还证明了两种木霉属物种均有效代谢3,4-DCA。最后，我们提供的证据表明，独立于NAT的转化仅（在T. virens中）或主要（在T. reesei中）负责观察到的3,4-DCA去除。我们得出的结论是，除了乙酰化作用之外，维尔纽斯氏梭菌和较小程度的里氏梭菌还可能利用另一种未知的代谢途径对AA进行解毒。这是木霉属植物中AA生物转化的第一个分子和功能表征。考虑到木霉菌具有清除污染土壤的潜力，这些结果揭示了真菌修复AA污染土壤的新可能性。

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