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首页> 外文期刊>Applied Microbiology >Newly Identified Thermostable Esterase from Sulfobacillus acidophilus: Properties and Performance in Phthalate Ester Degradation
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Newly Identified Thermostable Esterase from Sulfobacillus acidophilus: Properties and Performance in Phthalate Ester Degradation

机译：新鉴定的嗜酸硫杆菌热稳定酯酶：邻苯二甲酸酯降解的性质和性能

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摘要

EstS1, a newly identified thermostable esterase from Sulfobacillus acidophilus DSM10332, was heterologously expressed in Escherichia coli and shown to enzymatically degrade phthalate esters (PAEs) to their corresponding monoalkyl PAEs. The optimal pH and temperature of the esterase were found to be 8.0 and 70°C, respectively. The half-life of EstS1 at 60°C was 15 h, indicating that the enzyme had good thermostability. The specificity constant ( k _(cat)/ K_(m) ) of the enzyme for p -nitrophenyl butyrate was as high as 6,770 mM~(?1) s~(?1). The potential value of EstS1 was demonstrated by its ability to effectively hydrolyze 35 to 82% of PAEs (10 mM) within 2 min at 37°C, with all substrates being completely degraded within 24 h. At 60°C, the time required for complete hydrolysis of most PAEs was reduced by half. To our knowledge, this enzyme is a new esterase identified from thermophiles that is able to degrade various PAEs at high temperatures.
机译：EstS1是一种新发现的嗜酸嗜热芽孢杆菌DSM10332的热稳定酯酶，在大肠杆菌中异源表达，并显示可将邻苯二甲酸酯（PAE）酶解为相应的单烷基PAE。发现酯酶的最佳pH和温度分别为8.0和70℃。 EstS1在60°C的半衰期为15小时，表明该酶具有良好的热稳定性。该酶对丁酸对硝基苯酯的特异性常数（k_（cat）/ K_（m））高达6,770mM·（Δ1）s·（Δ1）。 EstS1的潜在价值通过其在37°C下2分钟内有效水解35％至82％的PAE（10 mM）的能力证明，所有底物在24 h内完全降解。在60°C下，大多数PAE完全水解所需的时间减少了一半。据我们所知，该酶是一种从嗜热菌中鉴定出的新酯酶，能够在高温下降解各种PAE。

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