• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Heterologous Expression of Lysergic Acid and Novel Ergot Alkaloids in Aspergillus fumigatus
【24h】

Heterologous Expression of Lysergic Acid and Novel Ergot Alkaloids in Aspergillus fumigatus

机译：烟曲霉中麦角碱和新型麦角生物碱的异源表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Different lineages of fungi produce distinct classes of ergot alkaloids. Lysergic acid-derived ergot alkaloids produced by fungi in the Clavicipitaceae are particularly important in agriculture and medicine. The pathway to lysergic acid is partly elucidated, but the gene encoding the enzyme that oxidizes the intermediate agroclavine is unknown. We investigated two candidate agroclavine oxidase genes from the fungus Epichlo? festucae var. lolii × Epichlo? typhina isolate Lp1 (henceforth referred to as Epichlo? sp. Lp1), which produces lysergic acid-derived ergot alkaloids. Candidate genes easH and cloA were expressed in a mutant strain of the mold Aspergillus fumigatus , which typically produces a subclass of ergot alkaloids not derived from agroclavine or lysergic acid. Candidate genes were coexpressed with the Epichlo? sp. Lp1 allele of easA , which encodes an enzyme that catalyzed the synthesis of agroclavine from an A. fumigatus intermediate; the agroclavine then served as the substrate for the candidate agroclavine oxidases. Strains expressing easA and cloA from Epichlo? sp. Lp1 produced lysergic acid from agroclavine, a process requiring a cumulative six-electron oxidation and a double-bond isomerization. Strains that accumulated excess agroclavine (as a result of Epichlo? sp. Lp1 easA expression in the absence of cloA ) metabolized it into two novel ergot alkaloids for which provisional structures were proposed on the basis of mass spectra and precursor feeding studies. Our data indicate that CloA catalyzes multiple reactions to produce lysergic acid from agroclavine and that combining genes from different ergot alkaloid pathways provides an effective strategy to engineer important pathway molecules and novel ergot alkaloids.
机译：真菌的不同谱系产生不同种类的麦角生物碱。锁骨科真菌产生的麦角酸麦角生物碱在农业和医学中尤为重要。麦角酸的途径已部分阐明，但尚不知道编码氧化中间农杆菌的酶的基因。我们调查了真菌Epichlo？的两个候选农锁酸氧化酶基因。费斯图卡河萝莉×Epichlo？鼠伤寒分离株Lp1（以下称为Epichlo？sp。Lp1），可产生麦角酸的麦角生物碱。候选基因easH和cloA在霉菌烟曲霉的突变株中表达，该突变株通常产生麦角生物碱的亚类，该麦角生物碱不是衍生自农锁酸或麦角酸。候选基因与Epichlo？ sp。 easA的Lp1等位基因，其编码一种酶，该酶催化烟曲霉中间产物合成农杆菌的合成;然后将农锁藤作为候选农锁藤氧化酶的底物。表达埃希氏菌的easA和cloA的菌株？ sp。 Lp1从农杆菌中产生麦角酸，该过程需要累积的六电子氧化和双键异构化。积累过量农锁骨藤的菌株（由于在不存在cloA的情况下Epichlo？sp。Lp1 easA表达的结果）将其代谢为两种新型麦角生物碱，并根据质谱和前体进料研究提出了其临时结构。我们的数据表明CloA催化了多种反应，从农杆菌中产生麦角酸，结合来自不同麦角生物碱途径的基因提供了一种有效的策略来工程化重要的途径分子和新型麦角生物碱。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号