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首页> 外文期刊>Applied Microbiology >pH Tolerance in Freshwater Bacterioplankton: Trait Variation of the Community as Measured by Leucine Incorporation
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pH Tolerance in Freshwater Bacterioplankton: Trait Variation of the Community as Measured by Leucine Incorporation

机译：淡水浮游细菌的pH耐受性：亮氨酸掺入法测量的社区的性状变化。

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摘要

pH is an important factor determining bacterial community composition in soil and water. We have directly determined the community tolerance (trait variation) to pH in communities from 22 lakes and streams ranging in pH from 4 to 9 using a growth-based method not relying on distinguishing between individual populations. The pH in the water samples was altered to up to 16 pH values, covering in situ pH ± 2.5 U, and the tolerance was assessed by measuring bacterial growth (Leu incorporation) instantaneously after pH adjustment. The resulting unimodal response curves, reflecting community tolerance to pH, were well modeled with a double logistic equation (mean R ~(2) = 0.97). The optimal pH for growth (pH_(opt)) among the bacterial communities was closely correlated with in situ pH, with a slope (0.89 ± 0.099) close to unity. The pH interval, in which growth was ≥90% of that at pH_(opt), was 1.1 to 3 pH units wide (mean 2.0 pH units). Tolerance response curves of communities originating from circum-neutral pH were symmetrical, whereas in high-pH (8.9) and especially in low-pH (<5.5) waters, asymmetric tolerance curves were found. In low-pH waters, decreasing pH was more detrimental for bacterial growth than increasing pH, with a tendency for the opposite for high-pH waters. A pH tolerance index, using the ratio of growth at only two pH values (pH 4 and 8), was closely related to pH_(opt) ( R ~(2) = 0.83), allowing for easy determination of pH tolerance during rapid changes in pH.
机译：pH是决定土壤和水中细菌群落组成的重要因素。我们使用基于增长的方法直接确定了22个湖泊和pH范围为4至9的溪流对pH的社区耐受性（性状变异），而无需依靠区分个体。将水样中的pH值更改为最多16个pH值，涵盖原位pH±2.5 U，并在调节pH后立即测量细菌的生长（Leu掺入）来评估耐受性。生成的反映社区对pH耐受性的单峰响应曲线用双逻辑方程很好地建模（平均R〜（2）= 0.97）。细菌群落中生长的最佳pH（pH_（opt））与原位pH密切相关，其斜率（0.89±0.099）接近于1。在pH值（opt）下生长≥90％的pH间隔宽为1.1至3个pH单位（平均2.0 pH单位）。源自环境中性pH的群落的耐受响应曲线是对称的，而在高pH（8.9）尤其是低pH（<5.5）的水中，发现了不对称的耐受曲线。在低pH值的水中，降低pH值比增加pH值更不利于细菌生长，而高pH值的趋势则相反。使用仅在两个pH值（pH 4和8）下的生长比例的pH耐受指数与pH_（opt）密切相关（R〜（2）= 0.83），可以轻松确定快速变化期间的pH耐受性在pH中。

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