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首页> 外文期刊>Applied Microbiology >Abundant Trimethylornithine Lipids and Specific Gene Sequences Are Indicative of Planctomycete Importance at the Oxic/Anoxic Interface in Sphagnum-Dominated Northern Wetlands
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Abundant Trimethylornithine Lipids and Specific Gene Sequences Are Indicative of Planctomycete Importance at the Oxic/Anoxic Interface in Sphagnum-Dominated Northern Wetlands

机译：丰富的Trimethylornithine脂质和特定的基因序列表明在以泥炭为主的北部湿地中，氧/无氧界面上的浮萍菌的重要性。

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Northern wetlands make up a substantial terrestrial carbon sink and are often dominated by decay-resistant Sphagnum mosses. Recent studies have shown that planctomycetes appear to be involved in degradation of Sphagnum -derived debris. Novel trimethylornithine (TMO) lipids have recently been characterized as abundant lipids in various Sphagnum wetland planctomycete isolates, but their occurrence in the environment has not yet been confirmed. We applied a combined intact polar lipid (IPL) and molecular analysis of peat cores collected from two northern wetlands (Saxn?s Mosse [Sweden] and Obukhovskoye [Russia]) in order to investigate the preferred niche and abundance of TMO-producing planctomycetes. TMOs were present throughout the profiles of Sphagnum bogs, but their concentration peaked at the oxic/anoxic interface, which coincided with a maximum abundance of planctomycete-specific 16S rRNA gene sequences. The sequences detected at the oxic/anoxic interface were affiliated with the Isosphaera group, while sequences present in the anoxic peat layers were related to an uncultured planctomycete group. Pyrosequencing-based analysis identified Planctomycetes as the major bacterial group at the oxic/anoxic interface at the Obukhovskoye peat (54% of total 16S rRNA gene sequence reads), followed by Acidobacteria (19% reads), while in the Saxn?s Mosse peat, Acidobacteria were dominant (46%), and Planctomycetes contributed to 6% of the total reads. The detection of abundant TMO lipids in planctomycetes isolated from peat bogs and the lack of TMO production by cultures of acidobacteria suggest that planctomycetes are the producers of TMOs in peat bogs. The higher accumulation of TMOs at the oxic/anoxic interface and the change in the planctomycete community with depth suggest that these IPLs could be synthesized as a response to changing redox conditions at the oxic/anoxic interface.
机译：北部湿地构成了一个重要的陆地碳汇，并且通常以抗腐烂的泥炭藓为主导。最近的研究表明，葡萄球菌似乎参与了泥炭藓衍生碎片的降解。新型三甲基鸟氨酸（TMO）脂质最近已被表征为多种泥炭湿地浮游菌分离物中的丰富脂质，但尚未在环境中证实它们的存在。我们应用完整的极性脂质（IPL）和从两个北部湿地（Saxn？s Mosse [瑞典]和Obukhovskoye [俄罗斯]）收集的泥炭芯的分子分析，以研究产生TMO的扁平菌的首选生态位和丰度。 TMOs存在于整个泥炭藓沼泽中，但是它们的浓度在有氧/缺氧界面达到峰值，这与最大的浮游菌特异16S rRNA基因序列吻合。在有氧/缺氧界面处检测到的序列与等位基因组相关，而在缺氧泥炭层中存在的序列与未培养的平生菌群相关。基于焦磷酸测序的分析确定了扁平菌是Obukhovskoye泥炭（占总16S rRNA基因序列读数的54％）在氧/缺氧界面的主要细菌群，其次是酸杆菌（19％读数），而在萨克森州的莫斯泥炭中，酸性杆菌占主导（46％），而浮游菌占总读数的6％。从泥炭沼泽分离的扁平菌中检测到丰富的TMO脂质，并且酸菌培养物缺乏TMO产生，这表明扁平菌是泥炭沼泽中TMO的产生者。 TMO在含氧/缺氧界面上的积累程度更高，且扁平菌群落的深度变化表明，这些IPL可以合成为对含氧/缺氧界面氧化还原条件变化的响应。

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