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首页> 外文期刊>Applied Microbiology >A 1,3-1,4-β-Glucan Utilization Regulon in Paenibacillus sp. Strain JDR-2
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A 1,3-1,4-β-Glucan Utilization Regulon in Paenibacillus sp. Strain JDR-2

机译：Paenibacillus sp。中的1,3-1,4-β-葡聚糖利用调节剂。应变JDR-2

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Paenibacillus sp. strain JDR-2 ( Paenibacillus JDR-2) secretes a multimodular cell-associated glycoside hydrolase family 10 (GH10) endoxylanase (XynA10A_(1)) that catalyzes the depolymerization of methylglucuronoxylan (MeGX_(n)) and rapidly assimilates the products of depolymerization. Efficient utilization of MeGX_(n) has been postulated to result from the coupling of the processes of exocellular depolymerization and assimilation of oligosaccharide products, followed by intracellular metabolism. Growth and substrate utilization patterns with barley glucan and laminarin similar to those observed with MeGX_(n) as a substrate suggest similar processes for 1,3-1,4-β-glucan and 1,3-β-glucan depolymerization and product assimilation. The Paenibacillus JDR-2 genome includes a cluster of genes encoding a secreted multimodular GH16 β-glucanase (Bgl16A_(1)) containing surface layer homology (SLH) domains, a secreted GH16 β-glucanase with only a catalytic domain (Bgl16A_(2)), transporter proteins, and transcriptional regulators. Recombinant Bgl16A_(1) and Bgl16A_(2) catalyze the formation of trisaccharides, tetrasaccharides, and larger oligosaccharides from barley glucan and of mono-, di-, tri-, and tetrasaccharides and larger oligosaccharides from laminarin. The lack of accumulation of depolymerization products during growth and a marked preference for polymeric glucan over depolymerization products support a process coupling extracellular depolymerization, assimilation, and intracellular metabolism for β-glucans similar to that ascribed to the GH10/GH67 xylan utilization system in Paenibacillus JDR-2. Coordinate expression of genes encoding GH16 β-glucanases, transporters, and transcriptional regulators supports their role as a regulon for the utilization of soluble β-glucans. As in the case of the xylan utilization regulons, this soluble β-glucan regulon provides advantages in the growth rate and yields on polymeric substrates and may be exploited for the efficient conversion of plant-derived polysaccharides to targeted products.
机译：芽孢杆菌菌株JDR-2（Paenibacillus JDR-2）分泌一种多模块细胞相关糖苷水解酶家族10（GH10）内切木聚糖酶（XynA10A_（1）），该酶催化甲基葡糖醛酸木聚糖（MeGX_（n））的解聚并迅速同化解聚产物。据推测，MeGX_（n）的有效利用是由于胞外解聚和寡糖产物同化过程的耦合，然后是细胞内代谢。大麦葡聚糖和层粘连蛋白的生长和底物利用模式与以MeGX_（n）为底物观察到的模式相似，表明1,3-1,4-β-葡聚糖和1,3-β-葡聚糖解聚和产物同化的过程相似。 Paenibacillus JDR-2基因组包括一组编码包含表面层同源性（SLH）结构域的分泌型多模块GH16β-葡聚糖酶（Bgl16A_（1）），仅具有催化结构域（Bgl16A_（2）的分泌型GH16β-葡聚糖酶的基因簇），转运蛋白和转录调节剂。重组Bgl16A_（1）和Bgl16A_（2）催化从大麦葡聚糖形成三糖，四糖和较大的寡糖，并从层粘连蛋白催化单糖，二糖，三糖和四糖以及较大的寡糖的形成。生长过程中缺乏解聚产物的积累，并且聚合葡聚糖明显优于解聚产物，这支持了偶联β-葡聚糖的细胞外解聚，同化和细胞内代谢的过程，这类似于Paenibacillus JDR中的GH10 / GH67木聚糖利用系统-2。编码GH16β-葡聚糖酶，转运蛋白和转录调节因子的基因的协调表达支持它们作为利用可溶性β-葡聚糖的调节子的作用。如在木聚糖利用调节剂的情况下，该可溶性β-葡聚糖调节剂在聚合物底物上的生长速率和产量方面提供优势，并且可以用于将植物来源的多糖有效转化为目标产物。

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