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首页> 外文期刊>Applied Microbiology >Sucrose- and Fructose-Specific Effects on the Transcriptome of Streptococcus mutans, as Determined by RNA Sequencing
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Sucrose- and Fructose-Specific Effects on the Transcriptome of Streptococcus mutans, as Determined by RNA Sequencing

机译：RNA测序确定的蔗糖和果糖对变形链球菌转录组的特异作用

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Recent genome-scale studies have begun to establish the scope and magnitude of the impacts of carbohydrate source and availability on the regulation of gene expression in bacteria. The effects of sugars on gene expression are particularly profound in a group of lactic acid bacteria that rely almost entirely on their saccharolytic activities for energy production and growth. For Streptococcus mutans , the major etiologic agent of human dental caries, sucrose is the carbohydrate that contributes in the most significant manner to establishment, persistence, and virulence of the organism. However, because this organism produces multiple extracellular sucrolytic enzymes that can release hexoses from sucrose, it has not been possible to study the specific effects of sucrose transport and metabolism on gene expression in the absence of carbohydrates that by themselves can elicit catabolite repression and induce expression of multiple genes. By employing RNA deep-sequencing (RNA-Seq) technology and mutants that lacked particular sucrose-metabolizing enzymes, we compared the transcriptomes of S. mutans bacteria growing on glucose, fructose, or sucrose as the sole carbohydrate source. The results provide a variety of new insights into the impact of sucrose transport and metabolism by S. mutans , including the likely expulsion of fructose after sucrose internalization and hydrolysis, and identify a set of genes that are differentially regulated by sucrose versus fructose. The findings significantly enhance our understanding of the genetics and physiology of this cariogenic pathogen.
机译：最近的基因组规模研究已经开始确定碳水化合物来源和可利用性对细菌基因表达的调控影响的范围和大小。糖对基因表达的影响在一组乳酸菌中尤为深刻，这些乳酸菌几乎完全依靠其糖酵解活性来产生和生长能量。对于人类龋齿的主要病因，变形链球菌来说，蔗糖是以最重要的方式有助于生物体的建立，持久性和致病性的碳水化合物。但是，由于这种生物体会产生多种可从蔗糖中释放出己糖的细胞外糖酵解酶，因此，在没有碳水化合物本身能引起分解代谢物抑制并诱导表达的碳水化合物的情况下，不可能研究蔗糖运输和代谢对基因表达的特定影响。多个基因。通过采用RNA深度测序（RNA-Seq）技术和缺少特定蔗糖代谢酶的突变体，我们比较了以葡萄糖，果糖或蔗糖作为唯一碳水化合物来源生长的变形链球菌细菌的转录组。结果为变形链球菌对蔗糖运输和代谢的影响提供了各种新见解，包括蔗糖内在化和水解后可能将果糖排出，并鉴定了一组受蔗糖和果糖差异调节的基因。这些发现大大增强了我们对这种致龋性病原体的遗传学和生理学的理解。

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