Engineering the meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum by Site Saturation Mutagenesis for d-Phenylalanine Synthesis

Xiuzhen Gao; Fang Huang; Jinhui Feng; Xi Chen; Hailing Zhang; Zhixiang Wang; Qiaqing Wu; Dunming Zhu

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Engineering the meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum by Site Saturation Mutagenesis for d-Phenylalanine Synthesis

机译：通过位点饱和诱变工程化嗜热共生细菌的内消旋二氨基庚二酸酯脱氢酶，用于合成d-苯丙氨酸

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In order to enlarge the substrate binding pocket of the meso -diaminopimelate dehydrogenase from Symbiobacterium thermophilum to accommodate larger 2-keto acids, four amino acid residues (Phe146, Thr171, Arg181, and His227) were targeted for site saturation mutagenesis. Among all mutants, the single mutant H227V had a specific activity of 2.39 ± 0.06 U · mg~(?1), which was 35.1-fold enhancement over the wild-type enzyme.

机译：为了扩大嗜热链球菌的内消旋二氨基庚二酸脱氢酶的底物结合口袋以容纳更大的2-酮酸，将四个氨基酸残基（Phe146，Thr171，Arg181和His227）用于位点饱和诱变。在所有突变体中，单个突变体H227V的比活为2.39±0.06 U·mg〜（？1），比野生型酶高35.1倍。

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《Applied Microbiology》 |2013年第16期|共4页
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Xiuzhen Gao; Fang Huang; Jinhui Feng; Xi Chen; Hailing Zhang; Zhixiang Wang; Qiaqing Wu; Dunming Zhu;
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1. Engineering the meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum by Site Saturation Mutagenesis for d-Phenylalanine Synthesis [J] . Xiuzhen Gao, Fang Huang, Jinhui Feng, Applied and Environmental Microbiology . 2013,第16期

机译：通过位点饱和诱变工程化嗜热共生细菌的内消旋二氨基庚二酸酯脱氢酶，用于合成d-苯丙氨酸
2. A Novel meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum: Overexpression, Characterization, and Potential for d-Amino Acid Synthesis [J] . Xiuzhen Gao, Xi Chen, Weidong Liu, Applied and Environmental Microbiology . 2012,第24期

机译：嗜热共生菌的新型中-二氨基庚二酸酯脱氢酶：d-氨基酸合成的过表达，表征和潜力
3. A Novel meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum: Overexpression, Characterization, and Potential for d-Amino Acid Synthesis [J] . Xiuzhen Gao, Xi Chen, Weidong Liu, Applied Microbiology . 2012,第24期

机译：嗜热共生菌的新型中-二氨基庚二酸酯脱氢酶：d-氨基酸合成的过表达，表征和潜力
4. Site-Directed Mutagenesis Study on the Thermal Stability of a Chimeric PQQ Glucose Dehydrogenase and Its Structural Interpretation [C] . ARIEF BUDI WITARTO, TAKAFUMI OHTERA, KOJI SODE Biotechnology for Fuels and Chemicals . 1999

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5. Probing the mechanism of saccharopine dehydrogenase from Saccharomyces cerevisiae using site-directed mutagenesis. [D] . Ekanayake, Devi Kosala. 2010

机译：使用定点诱变探讨酿酒酵母中糖醋栗脱氢酶的机理。
6. Engineering the meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum by Site Saturation Mutagenesis for d-Phenylalanine Synthesis [O] . Xiuzhen Gao, Fang Huang, Jinhui Feng, 2013

机译：通过位点饱和诱变工程化嗜热共生细菌的内消旋二氨基庚二酸脱氢酶用于合成d-苯丙氨酸
7. Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates [O] . Shuhong Mao, Jian-Wen Wang, Fufeng Liu, 2018

机译：基于3-酮甾体醇δ1-脱氢酶的部位导向饱和诱变的工程，用于甾体基材的有效生物转化的基于位点饱和诱变

Engineering the meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum by Site Saturation Mutagenesis for d-Phenylalanine Synthesis

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