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首页> 外文期刊>Applied Microbiology >Adherence to and Invasion of Human Intestinal Cells by Arcobacter Species and Their Virulence Genotypes
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Adherence to and Invasion of Human Intestinal Cells by Arcobacter Species and Their Virulence Genotypes

机译：变形杆菌对人肠道细胞的粘附和侵袭及其毒力基因型

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The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri , A. cryaerophilus , and A. skirrowii , as these have been associated with human cases of diarrhea, the probable transmission routes being through the ingestion of contaminated drinking water and food. To date, only limited studies of virulence traits in this genus have been undertaken. The present study used 60 Arcobacter strains isolated from different sources, representing 16 of the 17 species of the genus, to investigate their ability to adhere to and invade the human intestinal cell line Caco-2. In addition, the presence of five putative virulence genes ( ciaB , cadF , cj1349 , hecA , and irgA ) was screened for in these strains by PCR. All Arcobacter species except A. bivalviorum and Arcobacter sp. strain W63 adhered to Caco-2 cells, and most species (10/16) were invasive. The most invasive species were A. skirrowii , A. cryaerophilus , A. butzleri , and A. defluvii . All invasive strains were positive for ciaB (encoding a putative invasion protein). Other putative virulence genes were present in other species, i.e., A. butzleri ( cadF , cj1349 , irgA , and hecA ), A. trophiarum ( cj1349 ), A. ellisii ( cj1349 ), and A. defluvii ( irgA ). No virulence genes were detected in strains which showed little or no invasion of Caco-2 cells. These results indicate that many Arcobacter species are potential pathogens of humans and animals.
机译：弓形杆菌属由从各种来源分离出的17个物种组成。特别引起关注的是布氏曲霉，嗜冷曲霉和斯基氏曲霉，因为它们与人类腹泻有关，可能的传播途径是通过摄入被污染的饮用水和食物。迄今为止，仅对该属的毒力性状进行了有限的研究。本研究使用了60种从不同来源分离出的Arcobacter菌株，它们代表了该属17种中的16种，以研究其粘附和侵袭人类肠道细胞系Caco-2的能力。另外，通过PCR在这些菌株中筛选了五个推定的毒力基因（ciaB，cadF，cj1349，hecA和irgA）的存在。除双歧杆菌和双歧杆菌种以外的所有双歧杆菌种。 W63菌株粘附在Caco-2细胞上，大多数物种（10/16）具有侵袭性。最具入侵性的物种是短吻曲霉，嗜冷曲霉，酪but曲霉和德菲弧菌。所有侵入菌株均对ciaB（编码一种假定的侵入蛋白）呈阳性。其他推定的毒性基因也存在于其他物种中，例如布氏曲霉（cadF，cj1349，irgA和hecA），营养杆状菌（cj1349），艾氏拟南芥（cj1349）和德氏拟南芥（irgA）。在显示很少或没有侵袭Caco-2细胞的菌株中未检测到毒力基因。这些结果表明，许多杆菌属是人类和动物的潜在病原体。

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