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Articles of Significant Interest Selected from This Issue by the Editors

机译：编辑从本期中精选的重要文章

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Direct and simultaneous quantification of multiple pathogens has been desired for diagnostic and public health purposes for a long time. Using microfluidic technology, Ishii et al. (p. 28912898) developed a novel quantitative PCR system to quantify multiple enteric pathogens simultaneously. High-throughput quantitative information was obtained with sensitivity and specificity comparable to those of conventional quantitative PCR. This method was demonstrated to be applicable to environmental samples, such as fecal and water samples, and therefore should allow us to conduct more-reliable risk assessments than the current fecal contamination indicator approach.The archaea occupying extreme niches possess many unique metabolic pathways, which are being identified more and more recently. Studies by Han and colleagues (p. 29222931) demonstrated that four propionyl coenzyme A generation pathways, and particularly a novel 3-hydroxypropionate pathway, were simultaneously utilized for poly(3-hydroxybutyrate- co -3-hydroxyvalerate) (PHBV) biosynthesis in Haloferax mediterranei . This 3-hydroxypropionate pathway, coupling CO_(2) assimilation with biopolymer accumulation, may have great physiological importance for haloarchaea in carbon assimilation. This work has shed light on archaeal biology and carbon cycling in hypersaline ecosystems and may also provide novel strategies for PHBV engineering with controllable monomer composition.
机译：对于诊断和公共卫生目的，长期以来一直需要直接和同时定量多种病原体。使用微流体技术，Ishii等人。 （p。28912898）开发了一种新颖的定量PCR系统，可以同时定量多种肠道病原体。获得的高通量定量信息具有与传统定量PCR相当的灵敏度和特异性。已证明该方法适用于环境样品，例如粪便和水样，因此应使我们能够比目前的粪便污染指标方法进行更可靠的风险评估。占据极端生态位的古细菌具有许多独特的代谢途径，这些途径越来越多地被识别。 Han及其同事的研究（p。29222931）表明，同时利用了四个丙酰基辅酶A生成途径，特别是新的3-羟基丙酸酯途径，用于Haloferax中的聚（3-羟基丁酸酯-co -3-羟基戊酸酯）（PHBV）生物合成。地中海。这种3-羟基丙酸酯途径，耦合CO_（2）同化与生物聚合物的积累，可能对碳同化中的古生菌具有很大的生理重要性。这项工作揭示了高盐生态系统中的古生物学和碳循环，并且还可以为可控单体组成的PHBV工程提供新的策略。

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