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首页> 外文期刊>Applied Microbiology >Taxis of Pseudomonas putida F1 toward Phenylacetic Acid Is Mediated by the Energy Taxis Receptor Aer2
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Taxis of Pseudomonas putida F1 toward Phenylacetic Acid Is Mediated by the Energy Taxis Receptor Aer2

机译：能量出租车受体Aer2介导恶臭假单胞菌F1对苯乙酸的出租车。

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The phenylacetic acid (PAA) degradation pathway is a widely distributed funneling pathway for the catabolism of aromatic compounds, including the environmental pollutants styrene and ethylbenzene. However, bacterial chemotaxis to PAA has not been studied. The chemotactic strain Pseudomonas putida F1 has the ability to utilize PAA as a sole carbon and energy source. We identified a putative PAA degradation gene cluster ( paa ) in P. putida F1 and demonstrated that PAA serves as a chemoattractant. The chemotactic response was induced during growth with PAA and was dependent on PAA metabolism. A functional cheA gene was required for the response, indicating that PAA is sensed through the conserved chemotaxis signal transduction system. A P. putida F1 mutant lacking the energy taxis receptor Aer2 was deficient in PAA taxis, indicating that Aer2 is responsible for mediating the response to PAA. The requirement for metabolism and the role of Aer2 in the response indicate that P. putida F1 uses energy taxis to detect PAA. We also revealed that PAA is an attractant for Escherichia coli ; however, a mutant lacking a functional Aer energy receptor had a wild-type response to PAA in swim plate assays, suggesting that PAA is detected through a different mechanism in E. coli . The role of Aer2 as an energy taxis receptor provides the potential to sense a broad range of aromatic growth substrates as chemoattractants. Since chemotaxis has been shown to enhance the biodegradation of toxic pollutants, the ability to sense PAA gradients may have implications for the bioremediation of aromatic hydrocarbons that are degraded via the PAA pathway.
机译：苯乙酸（PAA）降解途径是一种广泛分布的漏斗途径，用于分解包括环境污染物苯乙烯和乙苯在内的芳族化合物。然而，尚未研究细菌对PAA的趋化性。趋化菌株恶臭假单胞菌F1具有利用PAA作为唯一碳和能源的能力。我们在恶臭假单胞菌F1中鉴定了一个假定的PAA降解基因簇（paa），并证明了PAA可以作为趋化剂。趋化反应在PAA的生长过程中被诱导，并且依赖于PAA的代谢。响应需要功能性cheA基因，表明PAA是通过保守的趋化性信号转导系统检测到的。缺少能量滑行受体Aer2的恶臭假单胞菌F1突变体在PAA滑行中不足，表明Aer2负责介导对PAA的应答。对新陈代谢的需求以及Aer2在反应中的作用表明恶臭假单胞菌F1使用能量滑行来检测PAA。我们还揭示了PAA是大肠杆菌的引诱剂;然而，在游泳板试验中，缺乏功能性Aer能量受体的突变体对PAA具有野生型反应，这表明在大肠杆菌中可通过不同的机制检测到PAA。 Aer2作为能量滑行受体的作用提供了潜在的潜力，可以感知广泛的芳香族生长底物作为化学吸引剂。由于已经显示趋化性增强了有毒污染物的生物降解，因此感知PAA梯度的能力可能会对通过PAA途径降解的芳烃的生物修复产生影响。

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