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首页> 外文期刊>Applied Microbiology >Abiotic Stress Resistance, a Novel Moonlighting Function of Ribosomal Protein RPL44 in the Halophilic Fungus Aspergillus glaucus
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Abiotic Stress Resistance, a Novel Moonlighting Function of Ribosomal Protein RPL44 in the Halophilic Fungus Aspergillus glaucus

机译：非生物胁迫抗性，核糖体蛋白RPL44在嗜盐真菌黑曲霉中的新月光功能

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摘要

Ribosomal proteins are highly conserved components of basal cellular organelles, primarily involved in the translation of mRNA leading to protein synthesis. However, certain ribosomal proteins moonlight in the development and differentiation of organisms. In this study, the ribosomal protein L44 (RPL44), associated with salt resistance, was screened from the halophilic fungus Aspergillus glaucus (AgRPL44), and its activity was investigated in Saccharomyces cerevisiae and Nicotiana tabacum . Sequence alignment revealed that AgRPL44 is one of the proteins of the large ribosomal subunit 60S. Expression of AgRPL44 was upregulated via treatment with salt, sorbitol, or heavy metals to demonstrate its response to osmotic stress. A homologous sequence from the model fungus Magnaporthe oryzae , MoRPL44, was cloned and compared with AgRPL44 in a yeast expression system. The results indicated that yeast cells with overexpressed AgRPL44 were more resistant to salt, drought, and heavy metals than were yeast cells expressing MoRPL44 at a similar level of stress. When AgRPL44 was introduced into M. oryzae , the transformants displayed obviously enhanced tolerance to salt and drought, indicating the potential value of AgRPL44 for genetic applications. To verify the value of its application in plants, tobacco was transformed with AgRPL44 , and the results were similar. Taken together, we conclude that AgRPL44 supports abiotic stress resistance and may have value for genetic application.
机译：核糖体蛋白是基底细胞器的高度保守的成分，主要参与导致蛋白质合成的mRNA的翻译。但是，某些核糖体蛋白在生物体的发育和分化中显得尤为重要。在这项研究中，从嗜盐真菌米曲霉（AgRPL44）中筛选了与盐抗性相关的核糖体蛋白L44（RPL44），并在酿酒酵母和烟草中研究了其活性。序列比对揭示AgRPL44是大核糖体亚基60S的蛋白质之一。通过用盐，山梨糖醇或重金属处理，AgRPL44的表达上调，以证明其对渗透压的响应。克隆了来自模型真菌Magnaporthe oryzae的同源序列MoRPL44，并在酵母表达系统中与AgRPL44进行了比较。结果表明，与在相似压力下表达MoRPL44的酵母细胞相比，过表达AgRPL44的酵母细胞对盐，干旱和重金属的抵抗力更高。将AgRPL44导入米曲霉后，转化株对盐和干旱的耐受性明显增强，表明AgRPL44在遗传应用中的潜在价值。为了验证其在植物中的应用价值，用AgRPL44转化了烟草，结果相似。两者合计，我们得出结论，AgRPL44支持非生物胁迫抗性，并可能具有遗传应用价值。

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