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首页> 外文期刊>Applied Microbiology >Lipopolysaccharide O-Chain Core Region Required for Cellular Cohesion and Compaction of In Vitro and Root Biofilms Developed by Rhizobium leguminosarum
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Lipopolysaccharide O-Chain Core Region Required for Cellular Cohesion and Compaction of In Vitro and Root Biofilms Developed by Rhizobium leguminosarum

机译：豆科根瘤菌开发的细胞内聚和紧实生物膜和根生物膜所需的脂多糖O链核心区

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The formation of biofilms is an important survival strategy allowing rhizobia to live on soil particles and plant roots. Within the microcolonies of the biofilm developed by Rhizobium leguminosarum , rhizobial cells interact tightly through lateral and polar connections, forming organized and compact cell aggregates. These microcolonies are embedded in a biofilm matrix, whose main component is the acidic exopolysaccharide (EPS). Our work shows that the O-chain core region of the R. leguminosarum lipopolysaccharide (LPS) (which stretches out of the cell surface) strongly influences bacterial adhesive properties and cell-cell cohesion. Mutants defective in the O chain or O-chain core moiety developed premature microcolonies in which lateral bacterial contacts were greatly reduced. Furthermore, cell-cell interactions within the microcolonies of the LPS mutants were mediated mostly through their poles, resulting in a biofilm with an altered three-dimensional structure and increased thickness. In addition, on the root epidermis and on root hairs, O-antigen core-defective strains showed altered biofilm patterns with the typical microcolony compaction impaired. Taken together, these results indicate that the surface-exposed moiety of the LPS is crucial for proper cell-to-cell interactions and for the formation of robust biofilms on different surfaces.
机译：生物膜的形成是重要的生存策略，可使根瘤菌生活在土壤颗粒和植物根系上。在豆科根瘤菌形成的生物膜的微菌落中，根瘤菌细胞通过侧向和极性连接紧密相互作用，形成有组织的致密细胞聚集体。这些微菌落嵌入生物膜基质中，其主要成分是酸性胞外多糖（EPS）。我们的工作表明，豆科动物根瘤菌脂多糖（LPS）的O链核心区域（延伸到细胞表面）强烈影响细菌的黏附特性和细胞间的凝聚力。 O链或O链核心部分有缺陷的突变体形成了过早的微菌落，其中横向细菌接触大大减少。此外，LPS突变体的微菌落内的细胞间相互作用主要是通过其极点介导的，从而导致生物膜的三维结构发生改变，厚度增加。另外，在根表皮和根毛上，O-抗原核心缺陷菌株显示出改变的生物膜模式，典型的微菌落压实受到损害。综上所述，这些结果表明，LPS的表面暴露部分对于适当的细胞间相互作用以及在不同表面上形成坚固的生物膜至关重要。

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