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首页> 外文期刊>Applied Microbiology >Mechanism for Restoration of Fertility in Hybrid Zygosaccharomyces rouxii Generated by Interspecies Hybridization
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Mechanism for Restoration of Fertility in Hybrid Zygosaccharomyces rouxii Generated by Interspecies Hybridization

机译：种间杂交产生的杂交鲁氏酵母的繁殖力恢复机制

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The mechanism of whole-genome duplication (WGD) in yeast has been intensively studied because it has a large impact on yeast evolution. WGD has shaped the genomic architecture of modern Saccharomyces cerevisiae; however, the mechanism for restoring fertility after interspecies hybridization, which would be involved in the process of WGD, has not been thoroughly elucidated. In this study, we obtained a draft genome sequence of the salt-tolerant yeast Zygosaccharomyces rouxii NBRC110957 and revealed that it is a hybrid lineage of Z. rouxii (allodiploid) with two subgenomes equivalent to NBRC1876. Because this allodiploid yeast can mate with other allodiploid strains and form spores, it can be a good model of restoring fertility after interspecies hybridization. We observed that NBRC110957 and NBRC1876 contain six mating-type-like (MTL) loci. There are no large deletions or deleterious mutations in MTL loci, except for several-base-pair deletions in the X region in certain MTL loci. We also assigned only one mating-type (MAT) locus that exclusively determines mating types from six MTL loci. These results suggest that it is possible to recover mating competence regardless of whether cells lose one MAT locus through random gene loss by mitotically dividing after interspecies hybridization. Moreover, we propose that perturbation of gene expression and substantial breakdown of MAT heterozygosity caused by chromosomal rearrangement at MTL loci play roles in restoring the mating competence of allodiploids. This scenario can provide a mechanism for restoring fertility after interspecies hybridization that is compatible with random gene loss models and suggests genomic plasticity during WGD in yeast.IMPORTANCE A whole-genome duplication occurred in an ancestor of the baker9s yeast Saccharomyces cerevisiae. The origins of this complex and multifaceted process, which requires intra- or interspecies hybridization followed by dysfunction of one mating-type (MAT) locus to regain mating competence, has not been thoroughly elucidated. In this study, we provide a mechanism for regaining fertility in an interspecies hybrid, Zygosaccharomyces rouxii. The draft genome sequence analysis and mating test showed that the Z. rouxii strain used in this study is an intact interspecies hybrid, suggesting that it is possible to recover fertility regardless of whether cells lose one MAT locus.
机译：酵母中全基因组复制（WGD）的机制已被深入研究，因为它对酵母的进化有很大影响。 WGD塑造了现代酿酒酵母的基因组结构。然而，尚未明确阐明种间杂交后恢复生育力的机制，而这种机制将参与WGD的过程。在这项研究中，我们获得了耐盐酵母鲁氏酵母（Zygosaccharomyces rouxii）NBRC110957的基因组序列草案，并揭示了它是鲁氏酵母（Z. rouxii）（异源二倍体）的杂交谱系，具有两个与NBRC1876相当的亚基因组。由于这种异源二倍体酵母可以与其他异源二倍体菌株交配并形成孢子，因此它可以成为种间杂交后恢复繁殖力的良好模型。我们观察到NBRC110957和NBRC1876包含六个交配型（MTL）基因座。 MTL基因座中没有大的缺失或有害突变，除了某些MTL基因座X区域中的几个碱基对缺失。我们还仅分配了一个交配类型（MAT）座位，该座位专门从六个MTL基因座确定交配类型。这些结果表明，不管种间杂交后细胞是否通过有丝分裂分裂而通过随机基因损失而失去一个MAT基因座，都可以恢复交配能力。此外，我们提出，基因表达的扰动和由MTL位点的染色体重排引起的MAT杂合性的实质性破坏在恢复异源二倍体的交配能力中起着作用。这种情况可以提供一种种间杂交后恢复生育力的机制，该机制与随机基因丢失模型兼容，并暗示了酵母在WGD期间的基因组可塑性。重要提示全基因组复制发生在baker9s酿酒酵母的祖先中。尚未彻底阐明这种复杂而多面的过程的起源，这种过程需要种内或种间杂交，然后需要一个交配型（MAT）基因座功能障碍才能恢复交配能力。在这项研究中，我们提供了一种在种间杂种鲁氏酵母（Zygosaccharomyces rouxii）中恢复生育力的机制。基因组序列草案分析和交配测试表明，该研究中使用的鲁氏沼虾菌株是完整的种间杂种，表明无论细胞是否失去一个MAT基因座，都可以恢复其育性。

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