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首页> 外文期刊>Applied Microbiology >ArcS, the Cognate Sensor Kinase in an Atypical Arc System of Shewanella oneidensis MR-1
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ArcS, the Cognate Sensor Kinase in an Atypical Arc System of Shewanella oneidensis MR-1

机译：ArcS，Sheidella oneidensis MR-1非典型电弧系统中的同源传感器激酶

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摘要

The availability of oxygen is a major environmental factor for many microbes, in particular for bacteria such as Shewanella species, which thrive in redox-stratified environments. One of the best-studied systems involved in mediating the response to changes in environmental oxygen levels is the Arc two-component system of Escherichia coli , consisting of the sensor kinase ArcB and the cognate response regulator ArcA. An ArcA ortholog was previously identified in Shewanella , and as in Escherichia coli , Shewanella ArcA is involved in regulating the response to shifts in oxygen levels. Here, we identified the hybrid sensor kinase SO_0577, now designated ArcS, as the previously elusive cognate sensor kinase of the Arc system in Shewanella oneidensis MR-1. Phenotypic mutant characterization, transcriptomic analysis, protein-protein interaction, and phosphotransfer studies revealed that the Shewanella Arc system consists of the sensor kinase ArcS, the single phosphotransfer domain protein HptA, and the response regulator ArcA. Phylogenetic analyses suggest that HptA might be a relict of ArcB. Conversely, ArcS is substantially different with respect to overall sequence homologies and domain organizations. Thus, we speculate that ArcS might have adopted the role of ArcB after a loss of the original sensor kinase, perhaps as a consequence of regulatory adaptation to a redox-stratified environment.
机译：氧气的可用性是许多微生物的主要环境因素，尤其是对于那些在氧化还原分层环境中繁衍生息的细菌，如希瓦氏菌。关于介导对环境氧水平变化的响应的研究最多的系统之一是大肠杆菌的Arc两组分系统，该系统由传感器激酶ArcB和关联响应调节剂ArcA组成。先前在希瓦氏菌中发现了ArcA直系同源物，就像在大肠杆菌中一样，希瓦氏菌ArcA参与调节对氧水平变化的响应。在这里，我们确定了杂种传感器激酶SO_0577（现称为ArcS）作为以前在Shewanella oneidensis MR-1中的Arc系统的关联传感器激酶。表型突变体表征，转录组分析，蛋白质-蛋白质相互作用和磷酸转移研究表明，希瓦氏菌Arc系统由传感器激酶ArcS，单个磷酸转移域蛋白HptA和响应调节剂ArcA组成。系统发育分析表明，HptA可能是ArcB的遗物。相反，ArcS在总体序列同源性和域组织方面有很大不同。因此，我们推测在丢失原始传感器激酶后，ArcS可能已经采用了ArcB的作用，这可能是由于对氧化还原分层环境的适应性调节的结果。

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