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首页> 外文期刊>Applied Microbiology >Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study
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Carriage and Fecal Counts of Cefotaxime M-Producing Escherichia coli in Pigs: a Longitudinal Study

机译：猪头孢噻肟M产生大肠杆菌的运输和粪便计数：一项纵向研究

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Current knowledge on extended-spectrum beta-lactamases (ESBLs) in animals is based largely on cross-sectional studies and qualitative data. The aim of this longitudinal study was to elucidate carriage proportions and fecal counts of ESBL-producing Escherichia coli in pigs during the production cycle. At each of three ESBL-positive single-sited farrow-to-finisher pig farms (farms A, B, and C) included in the study, individual fecal samples were taken from 17 to 20 sows 1 week before farrowing and from 2 piglets of each sow's litter four times from birth to slaughter (as piglets, weaners, and finishers). Cefotaxime (CTX)-resistant coliforms in feces were counted on MacConkey agar containing 2 μg/ml CTX and characterized for the presence of ESBL-encoding genes by PCR and sequencing. CTX-M-positive pigs were detected in all age groups at farms A ( bla _(CTX-M-9) group, compatible with bla _(CTX-M-14/17)) and B ( bla _(CTX-M-1) group, compatible with bla _(CTX-M-1/61)), whereas only three weaners were positive at farm C ( bla _(CTX-M-1) group, compatible with bla _(CTX-M-1/61)). A significant decrease in carriage was detected during the production cycle, with on average 50% carriage immediately after birth, 58% just before weaning, 29% during weaning, and 12% during finishing. The observed reduction in numbers of CTX-M-positive pigs was accompanied by a significant reduction in mean fecal counts of CTX-resistant coliforms from ～10~(7) CFU/g in piglets to ～10~(3) CFU/g in finishers ( P < 0.001). These findings provide novel information about the epidemiology of ESBLs at the farm level and have important implications for assessments of risks of meat contamination during slaughter.
机译：目前动物中广谱β-内酰胺酶（ESBLs）的知识主要基于横断面研究和定性数据。这项纵向研究的目的是阐明在生产周期中猪中产生ESBL的大肠杆菌的运输比例和粪便计数。在研究中包括的三个ESBL阳性单点产仔到肥育猪场（农场A，B和C）中，分别在产仔前1周从17至20头母猪采集了粪便样本，并从2头仔猪中采集了粪便样本。从出生到屠宰，每头母猪的产仔数为四次（如仔猪，断奶仔猪和育肥猪）。在包含2μg/ ml CTX的MacConkey琼脂上计数粪便中对头孢噻肟（CTX）的抗性大肠菌群，并通过PCR和测序对ESBL编码基因的存在进行表征。在农场A（bla _（CTX-M-9）组，与bla _（CTX-M-14 / 17）兼容）和B（bla _（CTX-M -1）组，与bla _（CTX-M-1 / 61）兼容，而农场C仅三个断奶仔鸡为阳性（bla _（CTX-M-1）组，与bla _（CTX-M-兼容） 1/61））。在生产周期中，运输量显着下降，出生后立即平均降低了50％，断奶前平均降低了58％，断奶期间降低了29％，育肥期间降低了12％。观察到的CTX-M阳性猪数量的减少伴随着CTX抗性大肠菌群粪便平均计数的显着降低，从仔猪的〜10〜（7）CFU / g降至仔猪的〜10〜（3）CFU / g。整理器（P <0.001）。这些发现提供了有关农场一级ESBLs流行病学的新颖信息，并对屠宰过程中肉类污染的风险评估具有重要意义。

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