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首页> 外文期刊>Applied Microbiology >Glycerol-3-Phosphate Acyltransferase Contributes to Triacylglycerol Biosynthesis, Lipid Droplet Formation, and Host Invasion in Metarhizium robertsii
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Glycerol-3-Phosphate Acyltransferase Contributes to Triacylglycerol Biosynthesis, Lipid Droplet Formation, and Host Invasion in Metarhizium robertsii

机译：甘油3-磷酸酰基转移酶有助于罗氏沼虾的三酰基甘油生物合成，脂质液滴形成和宿主入侵

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Enzymes involved in the triacylglycerol (TAG) biosynthesis have been well studied in the model organisms of yeasts and animals. Among these, the isoforms of glycerol-3-phosphate acyltransferase (GPAT) redundantly catalyze the first and rate-limiting step in glycerolipid synthesis. Here, we report the functions of mrGAT, a GPAT ortholog, in an insect-pathogenic fungus, Metarhizium robertsii . Unlike in yeasts and animals, a single copy of the mrGAT gene is present in the fungal genome and the gene deletion mutant is viable. Compared to the wild type and the gene-rescued mutant, the Δ mrGAT mutant demonstrated reduced abilities to produce conidia and synthesize TAG, glycerol, and total lipids. More importantly, we found that mrGAT is localized to the endoplasmic reticulum and directly linked to the formation of lipid droplets (LDs) in fungal cells. Insect bioassay results showed that mrGAT is required for full fungal virulence by aiding fungal penetration of host cuticles. Data from this study not only advance our understanding of GPAT functions in fungi but also suggest that filamentous fungi such as M. robertsii can serve as a good model to elucidate the role of the glycerol phosphate pathway in fungal physiology, particularly to determine the mechanistic connection of GPAT to LD formation.
机译：已经在酵母和动物的模型生物中对与三酰基甘油（TAG）生物合成有关的酶进行了充分的研究。其中，甘油3-磷酸酰基酰基转移酶（GPAT）的同工型多余地催化了甘油脂合成的第一步和限速步骤。在这里，我们报告mrGAT，GPAT直系同源物，在昆虫致病性真菌罗汉兰属（Metrhizium robertsii）中的功能。与酵母和动物不同，真菌基因组中存在单个副本的mrGAT基因，并且该基因缺失突变体是可行的。与野生型和基因拯救的突变体相比，ΔmrGAT突变体显示出降低分生孢子和合成TAG，甘油和总脂质的能力。更重要的是，我们发现mrGAT定位于内质网，并直接与真菌细胞中脂滴（LDs）的形成有关。昆虫生物测定结果表明，mrGAT通过帮助宿主表皮的真菌渗透来充分发挥真菌毒性。这项研究的数据不仅增进了我们对真菌中GPAT功能的理解，而且还表明丝状真菌（如罗氏支原体）可以作为阐明甘油磷酸途径在真菌生理学中的作用的良好模型，特别是确定机制连接GPAT对LD形成的影响。

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