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首页> 外文期刊>Applied Microbiology >Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica
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Identification and Upregulation of Biosynthetic Genes Required for Accumulation of Mycosporine-2-Glycine under Salt Stress Conditions in the Halotolerant Cyanobacterium Aphanothece halophytica

机译：盐胁迫条件下耐盐性蓝藻假单胞菌盐生植物中霉菌素-2-甘氨酸积累所需的生物合成基因的鉴定和上调。

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摘要

Mycosporine-like amino acids (MAAs) are valuable molecules that are the basis for important photoprotective constituents. Here we report molecular analysis of mycosporine-like amino acid biosynthetic genes from the halotolerant cyanobacterium Aphanothece halophytica , which can survive at high salinity and alkaline pH. This extremophile was found to have a unique MAA core (4-deoxygadusol)-synthesizing gene separated from three other genes. In vivo analysis showed accumulation of the mycosporine-2-glycine but not shinorine or mycosporine-glycine. Mycosporine-2-glycine accumulation was stimulated more under the stress condition of high salinity than UV-B radiation. The Aphanothece MAA biosynthetic genes also manifested a strong transcript level response to salt stress. Furthermore, the transformed Escherichia coli and Synechococcus strains expressing four putative Aphanothece MAA genes under the control of a native promoter were found to be capable of synthesizing mycosporine-2-glycine. The accumulation level of mycosporine-2-glycine was again higher under the high-salinity condition. In the transformed E. coli cells, its level was approximately 85.2 ± 0.7 μmol/g (dry weight). Successful production of a large amount of mycosporine in these cells provides a new opportunity in the search for an alternative natural sunscreen compound source.
机译：霉菌素样氨基酸（MAA）是有价值的分子，是重要的光保护成分的基础。在这里，我们报告了可从高盐度和碱性pH条件下存活的耐盐蓝藻细菌Aphanothece halophytica的霉菌素样氨基酸生物合成基因的分子分析。发现该极端微生物具有与其他三个基因分开的独特的MAA核心（4-deoxygadusol）合成基因。体内分析显示了霉菌素-2-甘氨酸的积累，但没有shinorine或霉菌素-甘氨酸的积累。在高盐度的胁迫条件下，霉菌素-2-甘氨酸的积累比UV-B辐射受到更多的刺激。 Aphanothece MAA生物合成基因还表现出对盐胁迫的强转录水平响应。此外，发现在天然启动子的控制下表达四个推定的Aphanothece MAA基因的转化的大肠杆菌和Synechococcus菌株能够合成霉菌素-2-甘氨酸。在高盐度条件下，霉菌素-2-甘氨酸的积累水平再次更高。在转化的大肠杆菌细胞中，其水平约为85.2±0.7μmol/ g（干重）。在这些细胞中成功生产大量霉菌素为寻找替代天然防晒化合物来源提供了新的机会。

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