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首页> 外文期刊>Applied Microbiology >Genes Similar to the Vibrio parahaemolyticus Virulence-Related Genes tdh, tlh, and vscC2 Occur in Other Vibrionaceae Species Isolated from a Pristine Estuary
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Genes Similar to the Vibrio parahaemolyticus Virulence-Related Genes tdh, tlh, and vscC2 Occur in Other Vibrionaceae Species Isolated from a Pristine Estuary

机译：与解毒弧菌毒力相关基因tdh，tlh和vscC2相似的基因发生在从原始河口分离的其他弧菌科物种中

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Detection of the human pathogen Vibrio parahaemolyticus often relies on molecular biological analysis of species-specific virulence factor genes. These genes have been employed in determinations of V. parahaemolyticus population numbers and the prevalence of pathogenic V. parahaemolyticus strains. Strains of the Vibrionaceae species Photobacterium damselae , Vibrio diabolicus , Vibrio harveyi , and Vibrio natriegens , as well as strains similar to Vibrio tubiashii , were isolated from a pristine salt marsh estuary. These strains were examined for the V. parahaemolyticus hemolysin genes tdh , trh , and tlh and for the V. parahaemolyticus type III secretion system 2α gene vscC2 using established PCR primers and protocols. Virulence-related genes occurred at high frequencies in non- V. parahaemolyticus Vibrionaceae species. V. diabolicus was of particular interest, as several strains were recovered, and the large majority (>83%) contained virulence-related genes. It is clear that detection of these genes does not ensure correct identification of virulent V. parahaemolyticus . Further, the occurrence of V. parahaemolyticus -like virulence factors in other vibrios potentially complicates tracking of outbreaks of V. parahaemolyticus infections.
机译：人类病原体副溶血性弧菌的检测通常依赖于物种特异性毒力因子基因的分子生物学分析。这些基因已被用于确定副溶血性弧菌的数量和致病性副溶血性弧菌菌株的流行。从原始盐沼河口分离出了弧菌科菌种：淡色弧菌，双弧菌，哈维氏弧菌和哈氏弧菌，以及与微管弧菌相似的菌株。使用已建立的PCR引物和方案，检查了这些菌株的副溶血弧菌溶血素基因tdh，trh和tlh，以及副溶血弧菌III型分泌系统2α基因vscC2。与毒力相关的基因在非溶血副弧菌弧菌科物种中高频率发生。双歧弧菌特别令人感兴趣，因为回收了几种菌株，并且绝大多数（> 83％）含有毒力相关基因。显然，检测这些基因不能确保正确鉴定有毒的副溶血性弧菌。此外，在其他弧菌中副溶血性弧菌样毒力因子的发生可能使追踪副溶血性弧菌感染的爆发变得复杂。

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