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首页> 外文期刊>Applied Microbiology >Sample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceans
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Sample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceans

机译：样品稀释度和细菌群落组成影响世界海洋地表水中经验性亮氨酸向碳的转化因子

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The transformation of leucine incorporation rates to prokaryotic carbon production rates requires the use of either theoretical or empirically determined conversion factors. Empirical leucine-to-carbon conversion factors (eCFs) vary widely across environments, and little is known about their potential controlling factors. We conducted 10 surface seawater manipulation experiments across the world's oceans, where the growth of the natural prokaryotic assemblages was promoted by filtration (i.e., removal of grazers [F treatment]) or filtration combined with dilution (i.e., also relieving resource competition [FD treatment]). The impact of sunlight exposure was also evaluated in the FD treatments, and we did not find a significant effect on the eCFs. The eCFs varied from 0.09 to 1.47 kg C mol Leu~(?1) and were significantly lower in the FD than in the F samples. Also, changes in bacterial community composition during the incubations, as assessed by automated ribosomal intergenic spacer analysis (ARISA), were more pronounced in the FD than in the F treatments, compared to unmanipulated controls. Thus, we discourage the common procedure of diluting samples (in addition to filtration) for eCF determination. The eCFs in the filtered treatment were negatively correlated with the initial chlorophyll a concentration, picocyanobacterial abundance (mostly Prochlorococcus ), and the percentage of heterotrophic prokaryotes with high nucleic acid content (%HNA). The latter two variables explained 80% of the eCF variability in the F treatment, supporting the view that both Prochlorococcus and HNA prokaryotes incorporate leucine in substantial amounts, although this results in relatively low carbon production rates in the oligotrophic ocean.
机译：亮氨酸掺入率向原核碳产率的转化需要使用理论或经验确定的转化因子。经验亮氨酸到碳的转化因子（eCF）在不同环境中差异很大，对其潜在控制因子知之甚少。我们在全球海洋中进行了10次表面海水处理实验，其中通过过滤（即，去除zer子[F处理]）或过滤与稀释相结合（即，也缓解了资源竞争[FD处理]）来促进天然原核生物的生长。 ]）。在FD处理中也评估了日光照射的影响，我们没有发现对eCF的显着影响。 eCFs从0.09到1.47 kg C mol Leu〜（？1）不等，FD中的eCFs明显低于F样品。而且，与未处理的对照相比，通过自动核糖体基因间间隔区分析（ARISA）评估的温育过程中细菌群落组成的变化在FD中比在F处理中更为明显。因此，我们不建议将样品稀释（除过滤以外）用于eCF测定的通用程序。过滤处理后的eCF与初始叶绿素a浓度，微蓝藻细菌丰度（主要是原绿球菌）以及具有高核酸含量（％HNA）的异养原核生物百分比呈负相关。后两个变量解释了F处理中80％的eCF变异性，支持了原绿球菌和HNA原核生物都大量掺入亮氨酸的观点，尽管这导致贫营养海洋中的碳生产率相对较低。

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