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首页> 外文期刊>Applied Microbiology >Escherichia coli Population Structure and Antibiotic Resistance at a Buffalo/Cattle Interface in Southern Africa
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Escherichia coli Population Structure and Antibiotic Resistance at a Buffalo/Cattle Interface in Southern Africa

机译：南部非洲水牛/牛界面的大肠杆菌种群结构和抗生素抗性

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At a human/livestock/wildlife interface, Escherichia coli populations were used to assess the risk of bacterial and antibiotic resistance dissemination between hosts. We used phenotypic and genotypic characterization techniques to describe the structure and the level of antibiotic resistance of E. coli commensal populations and the resistant Enterobacteriaceae carriage of sympatric African buffalo ( Syncerus caffer caffer ) and cattle populations characterized by their contact patterns in the southern part of Hwange ecosystem in Zimbabwe. Our results (i) confirmed our assumption that buffalo and cattle share similar phylogroup profiles, dominated by B1 (44.5%) and E (29.0%) phylogroups, with some variability in A phylogroup presence (from 1.9 to 12%); (ii) identified a significant gradient of antibiotic resistance from isolated buffalo to buffalo in contact with cattle and cattle populations expressed as the Murray score among Enterobacteriaceae (0.146, 0.258, and 0.340, respectively) and as the presence of tetracycline-, trimethoprim-, and amoxicillin-resistant subdominant E. coli strains (0, 5.7, and 38%, respectively); (iii) evidenced the dissemination of tetracycline, trimethoprim, and amoxicillin resistance genes ( tet , dfrA , and bla _(TEM-1)) in 26 isolated subdominant E. coli strains between nearby buffalo and cattle populations, that led us (iv) to hypothesize the role of the human/animal interface in the dissemination of genetic material from human to cattle and toward wildlife. The study of antibiotic resistance dissemination in multihost systems and at anthropizedatural interface is necessary to better understand and mitigate its multiple threats. These results also contribute to attempts aiming at using E. coli as a tool for the identification of pathogen transmission pathway in multihost systems.
机译：在人/畜/野生动物交界处，大肠杆菌种群被用来评估宿主之间细菌和抗生素耐药性传播的风险。我们使用表型和基因型表征技术来描述大肠杆菌共生种群的结构和水平以及同胞非洲水牛（Syncerus caffer caffer）和牛种群的耐药肠杆菌科细菌运输，其特征在于它们在南部南部的接触方式。津巴布韦的万基生态系统。我们的结果（i）证实了我们的假设，即水牛和牛具有相似的系统群特征，其中B1（44.5％）和E（29.0％）系统群占优势，A系统群存在一些差异（从1.9到12％）; （ii）确定了与牛和牛群接触的，从分离的水牛到水牛的抗生素耐药性的显着梯度，表示为肠杆菌科细菌的Murray评分（分别为0.146、0.258和0.340）以及四环素，甲氧苄氨嘧啶，和耐阿莫西林的主要大肠杆菌菌株（分别为0、5.7和38％）; （iii）证明四环素，甲氧苄啶和阿莫西林抗性基因（tet，dfrA和bla _（TEM-1））在附近水牛和牛群之间的26株分离的主要大肠杆菌菌株中传播，这导致了我们（iv）假设人/动物界面在遗传物质从人到牛以及向野生生物的传播中的作用。为了更好地理解和减轻其多重威胁，有必要在多宿主系统和人工/自然界面中研究抗生素耐药性的传播。这些结果也有助于尝试使用大肠杆菌作为鉴定多宿主系统中病原体传播途径的工具。

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