...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Effect of Earthworm Feeding Guilds on Ingested Dissimilatory Nitrate Reducers and Denitrifiers in the Alimentary Canal of the Earthworm
【24h】

Effect of Earthworm Feeding Guilds on Ingested Dissimilatory Nitrate Reducers and Denitrifiers in the Alimentary Canal of the Earthworm

机译：Feed饲喂协会对In消化道中异化硝酸盐还原剂和反硝化剂的吸收作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The earthworm gut is an anoxic nitrous oxide (N_(2)O)-emitting microzone in aerated soils. In situ conditions of the gut might stimulate ingested nitrate-reducing soil bacteria linked to this emission. The objective of this study was to determine if dissimilatory nitrate reducers and denitrifiers in the alimentary canal were affected by feeding guilds (epigeic [ Lumbricus rubellus ], anecic [ Lumbricus terrestris ], and endogeic [ Aporrectodea caliginosa ]). Genes and gene transcripts of narG (encodes a subunit of nitrate reductase and targets both dissimilatory nitrate reducers and denitrifiers) and nosZ (encodes a subunit of N_(2)O reductase and targets denitrifiers) were detected in guts and soils. Gut-derived sequences were similar to those of cultured and uncultured soil bacteria and to soil-derived sequences obtained in this study. Gut-derived narG sequences and narG terminal restriction fragments (TRFs) were affiliated mainly with Gram-positive organisms ( Actinobacteria ). The majority of gut- and uppermost-soil-derived narG transcripts were affiliated with Mycobacterium ( Actinobacteria ). In contrast, narG sequences indicative of Gram-negative organisms ( Proteobacteria ) were dominant in mineral soil. Most nosZ sequences and nosZ TRFs were affiliated with Bradyrhizobium ( Alphaproteobacteria ) and uncultured soil bacteria. TRF profiles indicated that nosZ transcripts were more affected by earthworm feeding guilds than were nosZ genes, whereas narG transcripts were less affected by earthworm feeding guilds than were narG genes. narG and nosZ transcripts were different and less diverse in the earthworm gut than in mineral soil. The collective results indicate that dissimilatory nitrate reducers and denitrifiers in the earthworm gut are soil derived and that ingested narG - and nosZ -containing taxa were not uniformly stimulated in the guts of worms from different feeding guilds.
机译：g肠是在充气土壤中发出一氧化二氮（N_（2）O）的微区。肠道的原位条件可能会刺激与这种排放有关的摄入的减少硝酸盐的土壤细菌的繁殖。这项研究的目的是确定消化道中的异化硝酸盐还原剂和反硝化剂是否受到饲喂行会的影响（上蜂[Lumbricus rubellus]，食欲动物[Lumbricus terrestris]和内耳[Aporrectodea caliginosa]）。在肠道和土壤中检测到了narG的基因和基因转录物（编码硝酸还原酶的一个亚基，同时靶向异化硝酸盐还原剂和反硝化剂）和nosZ（编码N_（2）O还原酶的一个亚单位，并靶向反硝化剂）。肠源序列与培养和未培养土壤细菌的序列相似，并且与本研究中获得的土壤源序列相似。肠源的narG序列和narG末端限制性片段（TRF）主要与革兰氏阳性生物（放线菌）相关。大部分源自肠道和最上层土壤的narG转录物都与分枝杆菌（Actinobacteria）相关。相反，指示革兰氏阴性生物（变形杆菌）的narG序列在矿质土壤中占主导地位。大多数nosZ序列和nosZ TRF都与缓生根瘤菌（Alphaproteobacteria）和未培养的土壤细菌有关。 TRF谱表明，nosZ转录物受nosZ基因的影响更大，而narG转录物受narG基因的影响较小。与矿物土壤相比，nar肠道中的narG和nosZ转录本不同且差异较小。总体结果表明，g肠道中的异化硝酸盐还原剂和反硝化剂是土壤来源的，来自不同饲养协会的蠕虫肠道中的纳尔和含​​nosZ的类群的摄入均未得到统一刺激。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号