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首页> 外文期刊>Applied Microbiology >Use of Flow Cytometry for Rapid, Quantitative Detection of Poliovirus-Infected Cells via TAT Peptide-Delivered Molecular Beacons
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Use of Flow Cytometry for Rapid, Quantitative Detection of Poliovirus-Infected Cells via TAT Peptide-Delivered Molecular Beacons

机译：流式细胞仪用于通过TAT肽传递的分子信标快速定量检测脊髓灰质炎病毒感染的细胞

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摘要

Rapid and efficient detection of viral infection is crucial for the prevention of disease spread during an outbreak and for timely clinical management. In this paper, the utility of Tat peptide-modified molecular beacons (MBs) as a rapid diagnostic tool for the detection of virus-infected cells was demonstrated. The rapid intracellular delivery mediated by the Tat peptide enabled the detection of infected cells within 30 s, reaching saturation in signal in 30 min. This rapid detection scheme was coupled with flow cytometry (FC), resulting in an automated, high-throughput method for the identification of virus-infected cells. Because of the 2-order-of-magnitude difference in fluorescence intensity between infected and uninfected cells, as few as 1% infected cells could be detected. Because of its speed and sensitivity, this approach may be adapted for the practical diagnosis of multiple viral infections.
机译：快速有效地检测病毒感染对于预防疾病暴发期间的疾病传播以及及时进行临床管理至关重要。在本文中，证明了Tat肽修饰的分子信标（MBs）作为检测病毒感染细胞的快速诊断工具的实用性。 Tat肽介导的细胞内快速递送能够在30 s内检测到感染的细胞，并在30分钟内达到信号饱和。这种快速检测方案与流式细胞仪（FC）结合使用，从而形成了一种自动的高通量方法，用于鉴定病毒感染的细胞。由于感染细胞和未感染细胞之间荧光强度的2个数量级差异，因此只能检测到1％的感染细胞。由于其速度和敏感性，该方法可适用于多种病毒感染的实际诊断。

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