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首页> 外文期刊>Applied Microbiology >Roles of Alkyl Hydroperoxide Reductase Subunit C (AhpC) in Viable but Nonculturable Vibrio parahaemolyticus
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Roles of Alkyl Hydroperoxide Reductase Subunit C (AhpC) in Viable but Nonculturable Vibrio parahaemolyticus

机译：烷基氢过氧化物还原酶亚基C（AhpC）在可行但不可培养的副溶血性弧菌中的作用

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Alkyl hydroperoxide reductase subunit C (AhpC) is the catalytic subunit responsible for the detoxification of reactive oxygen species that form in bacterial cells or are derived from the host; thus, AhpC facilitates the survival of pathogenic bacteria under environmental stresses or during infection. This study investigates the role of AhpC in the induction and maintenance of a viable but nonculturable (VBNC) state in Vibrio parahaemolyticus . In this investigation, ahpC1 (VPA1683) and ahpC2 (VP0580) were identified in chromosomes II and I of this pathogen, respectively. Mutants with deletions of these two ahpC genes and their complementary strains were constructed from the parent strain KX-V231. The growth of these strains was monitored on tryptic soy agar–3% NaCl in the presence of the extrinsic peroxides H_(2)O_(2) and tert -butyl hydroperoxide ( t -BOOH) at different incubation temperatures. The results revealed that both ahpC genes were protective against t -BOOH, while ahpC1 was protective against H_(2)O_(2). The protective function of ahpC2 at 4°C was higher than that of ahpC1 . The times required to induce the VBNC state (4.7 weeks) at 4°C in a modified Morita mineral salt solution with 0.5% NaCl and then to maintain the VBNC state (4.7 weeks) in an ahpC2 mutant and an ahpC1 ahpC2 double mutant were significantly shorter than those for the parent strain (for induction, 6.2 weeks; for maintenance, 7.8 weeks) and the ahpC1 mutant (for induction, 6.0 weeks; for maintenance, 8.0 weeks) ( P < 0.03). Complementation with an ahpC2 gene reversed the effects of the ahpC2 mutation in shortening the times for induction and maintenance of the VBNC state. This investigation identified the different functions of the two ahpC genes and confirmed the particular role of ahpC2 in the VBNC state of V. parahaemolyticus .
机译：烷基氢过氧化物还原酶亚基C（AhpC）是催化亚基，负责对细菌细胞中形成的或源自宿主的活性氧进行解毒。因此，AhpC促进了在环境压力下或感染过程中病原细菌的存活。这项研究调查了AhpC在副溶血性弧菌中诱导和维持一个可行但不可培养（VBNC）状态的作用。在这项研究中，分别在该病原体的染色体II和I中鉴定了ahpC1（VPA1683）和ahpC2（VP0580）。从亲本菌株KX-V231构建具有这两个ahpC基因及其互补菌株缺失的突变体。在不同的孵育温度下，在存在外源性过氧化物H_（2）O_（2）和叔丁基过氧化氢（t-BOOH）的情况下，在胰蛋白酶大豆琼脂3％NaCl上监测这些菌株的生长。结果表明，两个ahpC基因均对t -BOOH具有保护作用，而ahpC1基因则对H_（2）O_（2）具有保护作用。 ahpC2在4°C时的保护功能高于ahpC1。在含有0.5％NaCl的改良Morita矿物盐溶液中于4°C诱导VBNC状态（4.7周），然后在ahpC2突变体和ahpC1 ahpC2双突变体中维持VBNC状态（4.7周）所需的时间显着比亲本菌株（诱导6.2周;维持7.8周）和ahpC1突变体（诱导6.0周;维持8.0周）短（P <0.03）。与ahpC2基因互补可逆转ahpC2突变的影响，从而缩短了诱导和维持VBNC状态的时间。这项调查确定了两个ahpC基因的不同功能，并确认了ahpC2在副溶血弧菌的VBNC状态中的特殊作用。

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