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首页> 外文期刊>Applied Microbiology >Mechanisms of Human Adenovirus Inactivation by Sunlight and UVC Light as Examined by Quantitative PCR and Quantitative Proteomics
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Mechanisms of Human Adenovirus Inactivation by Sunlight and UVC Light as Examined by Quantitative PCR and Quantitative Proteomics

机译：定量PCR和定量蛋白质组学研究的阳光和UVC光灭活人类腺病毒的机制

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Human adenoviruses (HAdV) are important pathogens in both industrialized and developing nations. HAdV has been shown to be relatively resistant to monochromatic UVC light. Polychromatic UVC light, in contrast, is a more effective means of disinfection, presumably due to the involvement of viral proteins in the inactivation mechanism. Solar disinfection of HAdV, finally, is only poorly understood. In this paper, the kinetics and mechanism of HAdV inactivation by UVC light and direct and indirect solar disinfection are elucidated. PCR and mass spectrometry were employed to quantify the extent of genome and protein degradation and to localize the affected regions in the HAdV proteins. For this purpose, we used for the first time an approach involving stable isotope labeling by amino acids in cell culture (SILAC) of a human virus. Inactivation by UVC light and the full sunlight spectrum were found to efficiently inactivate HAdV, whereas UVA-visible light only caused inactivation in the presence of external sensitizers (indirect solar disinfection). Genome damage was significant for UVC but was less important for solar disinfection. In contrast, indirect solar disinfection exhibited extensive protein degradation. In particular, the fiber protein and the amino acids responsible for host binding within the fiber protein were shown to degrade. In addition, the central domain of the penton protein was damaged, which may inhibit interactions with the fiber protein and lead to a disruption of the initial stages of infection. Damage to the hexon protein, however, appeared to affect only regions not directly involved in the infectious cycle.
机译：人腺病毒（HAdV）在工业化国家和发展中国家都是重要的病原体。 HAdV已显示出相对抗单色UVC光的能力。相比之下，多色UVC光是一种更有效的消毒方法，大概是由于病毒蛋白参与了灭活机制。最后，对HAdV的太阳能消毒知之甚少。本文阐明了UVC光灭活HAdV的动力学和机理，以及直接和间接的日光消毒。 PCR和质谱法用于定量基因组和蛋白质降解的程度，并定位HAdV蛋白质中的受影响区域。为此，我们首次使用了一种涉及通过人类病毒细胞培养（SILAC）中的氨基酸进行稳定同位素标记的方法。发现被UVC光灭活和整个太阳光光谱有效灭活HAdV，而可见UVA的光仅在存在外部敏化剂的情况下才引起灭活（间接太阳消毒）。基因组损伤对UVC而言很重要，但对日光消毒而言则不太重要。相反，间接太阳消毒表现出广泛的蛋白质降解。特别地，显示纤维蛋白和负责纤维蛋白内的宿主结合的氨基酸降解。另外，戊烯蛋白的中央结构域被破坏，这可能抑制与纤维蛋白的相互作用并导致感染初始阶段的破坏。然而，六邻体蛋白的损害似乎仅影响不直接涉及感染周期的区域。

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