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首页> 外文期刊>Applied Microbiology >Transcriptomic Responses of Phanerochaete chrysosporium to Oak Acetonic Extracts: Focus on a New Glutathione Transferase
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Transcriptomic Responses of Phanerochaete chrysosporium to Oak Acetonic Extracts: Focus on a New Glutathione Transferase

机译：Phanerochaete chrysosporium对橡树醋酸提取物的转录反应：集中在一个新的谷胱甘肽转移酶。

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The first steps of wood degradation by fungi lead to the release of toxic compounds known as extractives. To better understand how lignolytic fungi cope with the toxicity of these molecules, a transcriptomic analysis of Phanerochaete chrysosporium genes was performed in the presence of oak acetonic extracts. It reveals that in complement to the extracellular machinery of degradation, intracellular antioxidant and detoxification systems contribute to the lignolytic capabilities of fungi, presumably by preventing cellular damages and maintaining fungal health. Focusing on these systems, a glutathione transferase ( P. chrysosporium GTT2.1 [PcGTT2.1]) has been selected for functional characterization. This enzyme, not characterized so far in basidiomycetes, has been classified first as a GTT2 compared to the Saccharomyces cerevisiae isoform. However, a deeper analysis shows that the GTT2.1 isoform has evolved functionally to reduce lipid peroxidation by recognizing high-molecular-weight peroxides as substrates. Moreover, the GTT2.1 gene has been lost in some non-wood-decay fungi. This example suggests that the intracellular detoxification system evolved concomitantly with the extracellular ligninolytic machinery in relation to the capacity of fungi to degrade wood.
机译：木材被真菌降解的第一步导致释放有毒化合物，称为提取物。为了更好地理解木质素分解真菌如何应对这些分子的毒性，在橡木丙酮提取物存在的情况下，对Phanerochaete chrysosporium基因进行了转录组分析。它揭示了补充降解的细胞外机制，细胞内抗氧化剂和解毒系统有助于真菌的木质素分解能力，大概是通过防止细胞损伤并保持真菌健康。着眼于这些系统，已经选择了谷胱甘肽转移酶（P. chrysosporium GTT2.1 [PcGTT2.1]）进行功能表征。与酿酒酵母同工型相比，迄今尚未在担子菌中表征的该酶首先被分类为GTT2。但是，更深入的分析表明，GTT2.1亚型通过识别高分子量过氧化物为底物，已在功能上得以发展，以减少脂质过氧化。此外，GTT2.1基因已经在一些非木材腐烂真菌中丢失。该实施例表明，在真菌降解木材的能力方面，细胞内解毒系统与细胞外木质素分解机制同时发展。

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