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首页> 外文期刊>Applied Microbiology >Biomineralization of Uranium by PhoY Phosphatase Activity Aids Cell Survival in Caulobacter crescentus
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Biomineralization of Uranium by PhoY Phosphatase Activity Aids Cell Survival in Caulobacter crescentus

机译：PhoY磷酸酶活性使铀生物矿化有助于新月形杆菌的细胞存活。

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Caulobacter crescentus is known to tolerate high levels of uranium [U(VI)], but its detoxification mechanism is poorly understood. Here we show that C. crescentus is able to facilitate U(VI) biomineralization through the formation of U-P_(i) precipitates via its native alkaline phosphatase activity. The U-P_(i) precipitates, deposited on the cell surface in the form of meta-autunite structures, have a lower U/P_(i) ratio than do chemically produced precipitates. The enzyme that is responsible for the phosphatase activity and thus the biomineralization process is identified as PhoY, a periplasmic alkaline phosphatase with broad substrate specificity. Furthermore, PhoY is shown to confer a survival advantage on C. crescentus toward U(VI) under both growth and nongrowth conditions. Results obtained in this study thus highlight U(VI) biomineralization as a resistance mechanism in microbes, which not only improves our understanding of bacterium-mineral interactions but also aids in defining potential ecological niches for metal-resistant bacteria.
机译：众所周知，新月形杆菌可以耐受高水平的铀[U（VI）]，但人们对其排毒机制的了解却很少。在这里，我们显示C.crescentus能够通过其天然碱性磷酸酶活性通过形成U-P_（i）沉淀物来促进U（VI）生物矿化。 U-P_（i）沉淀物以亚纯金铁矿结构的形式沉积在细胞表面，其U / P_（i）比值比化学产生的沉淀物低。负责磷酸酶活性和生物矿化过程的酶被鉴定为PhoY，一种具有广泛的底物特异性的周质碱性磷酸酶。此外，PhoY已显示在生长和不生长条件下均能使新月形梭菌对U（VI）具有生存优势。因此，在这项研究中获得的结果突出了U（VI）生物矿化作用作为微生物的抗药性机制，这不仅增进了我们对细菌-矿物质相互作用的理解，而且还有助于确定抗金属细菌的潜在生态位。

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