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首页> 外文期刊>Applied Microbiology >Characterization of a Planctomycetal Organelle: a Novel Bacterial Microcompartment for the Aerobic Degradation of Plant Saccharides
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Characterization of a Planctomycetal Organelle: a Novel Bacterial Microcompartment for the Aerobic Degradation of Plant Saccharides

机译：扁平菌的细胞器的表征：一种新型的细菌微隔室，对植物糖有氧降解

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Bacterial microcompartments (BMCs) are organelles that encapsulate functionally linked enzymes within a proteinaceous shell. The prototypical example is the carboxysome, which functions in carbon fixation in cyanobacteria and some chemoautotrophs. It is increasingly apparent that diverse heterotrophic bacteria contain BMCs that are involved in catabolic reactions, and many of the BMCs are predicted to have novel functions. However, most of these putative organelles have not been experimentally characterized. In this study, we sought to discover the function of a conserved BMC gene cluster encoded in the majority of the sequenced planctomycete genomes. This BMC is especially notable for its relatively simple genetic composition, its remote phylogenetic position relative to characterized BMCs, and its apparent exclusivity to the enigmatic Verrucomicrobia and Planctomycetes . Members of the phylum Planctomycetes are known for their morphological dissimilarity to the rest of the bacterial domain: internal membranes, reproduction by budding, and lack of peptidoglycan. As a result, they are ripe for many discoveries, but currently the tools for genetic studies are very limited. We expanded the genetic toolbox for the planctomycetes and generated directed gene knockouts of BMC-related genes in Planctomyces limnophilus . A metabolic activity screen revealed that BMC gene products are involved in the degradation of a number of plant and algal cell wall sugars. Among these sugars, we confirmed that BMCs are formed and required for growth on l-fucose and l-rhamnose. Our results shed light on the functional diversity of BMCs as well as their ecological role in the planctomycetes, which are commonly associated with algae.
机译：细菌微区室（BMC）是将功能性连接的酶封装在蛋白质壳内的细胞器。典型的例子是羧基小体，它在蓝细菌和某些化学自养生物的碳固定中起作用。越来越明显的是，各种各样的异养细菌都含有参与分解代谢反应的BMC，并且许多BMC被预测具有新颖的功能。但是，大多数这些推定的细胞器尚未进行实验表征。在这项研究中，我们试图发现保守的BMC基因簇的功能，该簇编码在大多数测序的扁平菌基因组中。这种BMC以其相对简单的遗传组成，相对于特征性BMC的遥远系统发育位置以及其对神秘的疣状微生物和扁平菌的明显排他性而特别引人注目。扁平菌门的成员以其与其余细菌域的形态学差异而著称：内膜，通过萌芽繁殖和缺乏肽聚糖。结果，它们已经为许多发现所成熟，但是目前用于遗传研究的工具非常有限。我们扩展了扁平菌的遗传工具箱，并在嗜线菌中生成了BMC相关基因的定向基因敲除。代谢活性筛选显示BMC基因产物与许多植物和藻类细胞壁糖的降解有关。在这些糖中，我们证实了BMC在L-岩藻糖和L-鼠李糖上的形成和生长所需。我们的研究结果揭示了BMC的功能多样性以及它们在通常与藻类有关的扁平菌中的生态作用。

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