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首页> 外文期刊>Applied Microbiology >The Cytosolic pH of Individual Saccharomyces cerevisiae Cells Is a Key Factor in Acetic Acid Tolerance
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The Cytosolic pH of Individual Saccharomyces cerevisiae Cells Is a Key Factor in Acetic Acid Tolerance

机译：单个酿酒酵母细胞的胞质pH是耐乙酸性的关键因素

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It was shown recently that individual cells of an isogenic Saccharomyces cerevisiae population show variability in acetic acid tolerance, and this variability affects the quantitative manifestation of the trait at the population level. In the current study, we investigated whether cell-to-cell variability in acetic acid tolerance could be explained by the observed differences in the cytosolic pHs of individual cells immediately before exposure to the acid. Results obtained with cells of the strain CEN.PK113-7D in synthetic medium containing 96 mM acetic acid (pH 4.5) showed a direct correlation between the initial cytosolic pH and the cytosolic pH drop after exposure to the acid. Moreover, only cells with a low initial cytosolic pH, which experienced a less severe drop in cytosolic pH, were able to proliferate. A similar correlation between initial cytosolic pH and cytosolic pH drop was also observed in the more acid-tolerant strain MUCL 11987-9. Interestingly, a fraction of cells in the MUCL 11987-9 population showed initial cytosolic pH values below the minimal cytosolic pH detected in cells of the strain CEN.PK113-7D; consequently, these cells experienced less severe drops in cytosolic pH. Although this might explain in part the difference between the two strains with regard to the number of cells that resumed proliferation, it was observed that all cells from strain MUCL 11987-9 were able to proliferate, independently of their initial cytosolic pH. Therefore, other factors must also be involved in the greater ability of MUCL 11987-9 cells to endure strong drops in cytosolic pH.
机译：最近显示，同基因酿酒酵母群体的单个细胞显示出乙酸耐受性的变异性，并且这种变异性在种群水平上影响该性状的定量表现。在当前的研究中，我们调查了乙酸耐受性的细胞间差异是否可以通过在暴露于酸之前单个细胞的胞浆pH值的观察到的差异来解释。在含有96 mM乙酸（pH 4.5）的合成培养基中，菌株CEN.PK113-7D的细胞获得的结果显示，初始胞质pH与暴露于该酸后的胞质pH下降之间具有直接相关性。此外，只有具有较低的初始胞质pH的细胞能够增殖，而这些细胞的初始胞质pH值降低的幅度较小。在更耐酸的菌株MUCL 11987-9中也观察到了初始细胞质pH和细胞质pH下降之间的相似相关性。有趣的是，MUCL 11987-9群体中的一小部分细胞显示的初始胞质pH值低于菌株CEN.PK113-7D的细胞中检测到的最小胞质pH。因此，这些细胞的胞浆pH下降幅度较小。尽管这可以部分解释两种菌株之间关于恢复增殖的细胞数量的差异，但是可以观察到，来自菌株MUCL 11987-9的所有细胞都能够增殖，而与它们的初始胞质pH值无关。因此，MUCL 11987-9细胞具有更大的耐受细胞质pH下降的能力，还必须涉及其他因素。

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