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首页> 外文期刊>Applied Microbiology >Differences in Physical and Biochemical Properties of Thermus scotoductus SA-01 Cultured with Dielectric or Convection Heating
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Differences in Physical and Biochemical Properties of Thermus scotoductus SA-01 Cultured with Dielectric or Convection Heating

机译：介电或对流加热培养的Thermus scotoductus SA-01的理化特性差异

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A thermophile, Thermus scotoductus SA-01, was cultured within a constant-temperature (65°C) microwave (MW) digester to determine if MW-specific effects influenced the growth and physiology of the organism. As a control, T. scotoductus cells were also cultured using convection heating at the same temperature as the MW studies. Cell growth was analyzed by optical density (OD) measurements, and cell morphologies were characterized using electron microscopy imaging (scanning electron microscopy [SEM] and transmission electron microscopy [TEM]), dynamic light scattering (DLS), and atomic force microscopy (AFM). Biophysical properties (i.e., turgor pressure) were also calculated with AFM, and biochemical compositions (i.e., proteins, nucleic acids, fatty acids) were analyzed by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. Gas chromatography-mass spectrometry (GC-MS) was used to analyze the fatty acid methyl esters extracted from cell membranes. Here we report successful cultivation of a thermophile with only dielectric heating. Under the MW conditions for growth, cell walls remained intact and there were no indications of membrane damage or cell leakage. Results from these studies also demonstrated that T. scotoductus cells grown with MW heating exhibited accelerated growth rates in addition to altered cell morphologies and biochemical compositions compared with oven-grown cells.
机译：在恒温（65°C）微波（MW）消化器中培养嗜热菌Thermus scotoductus SA-01，以确定MW特异性作用是否影响了生物的生长和生理。作为对照，还使用对流加热在与MW研究相同的温度下培养了T. scotoductus细胞。细胞生长通过光密度（OD）测量进行分析，并使用电子显微镜成像（扫描电子显微镜[SEM]和透射电子显微镜[TEM]），动态光散射（DLS）和原子力显微镜（AFM）对细胞形态进行表征）。还用AFM计算生物物理性质（即，膨胀压力），并通过衰减全反射-傅里叶变换红外（ATR-FTIR）光谱分析生化组成（即，蛋白质，核酸，脂肪酸）。气相色谱-质谱（GC-MS）用于分析从细胞膜中提取的脂肪酸甲酯。在这里，我们报告仅用电介质加热就成功地培育了嗜热菌。在MW生长条件下，细胞壁保持完整，没有膜损伤或细胞渗漏的迹象。这些研究的结果还表明，与烤箱培养的细胞相比，MW加热培养的T. scotoductus细胞除了改变了细胞的形态和生化成分外，还显示出加速的生长速率。

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