...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Multilocus Sequence Analysis Reveals Three Distinct Populations of “Candidatus Phytoplasma palmicola” with a Specific Geographical Distribution on the African Continent
【24h】

Multilocus Sequence Analysis Reveals Three Distinct Populations of “Candidatus Phytoplasma palmicola” with a Specific Geographical Distribution on the African Continent

机译：多基因座序列分析揭示了非洲大陆上三个特定地理分布的“ Candidatus Phytoplasma palmicola”不同种群。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To sustain epidemiological studies on coconut lethal yellowing disease (CLYD), a devastating disease in Africa caused by a phytoplasma, we developed a multilocus sequence typing (MLST) scheme for “Candidatus Phytoplasma palmicola” based on eight housekeeping genes. At the continental level, eight different sequence types were identified among 132 “Candidatus Phytoplasma palmicola”-infected coconuts collected in Ghana, Nigeria, and Mozambique, where CLYD epidemics are still very active. “Candidatus Phytoplasma palmicola” appeared to be a bacterium that is subject to strong bottlenecks, reducing the fixation of positively selected beneficial mutations into the bacterial population. This phenomenon, as well as a limited plant host range, might explain the observed country-specific distribution of the eight haplotypes. As an alternative means to increase fitness, bacteria can also undergo genetic exchange; however, no evidence for such recombination events was found for “Candidatus Phytoplasma palmicola.” The implications for CLYD epidemiology and prophylactic control are discussed. The usefulness of seven housekeeping genes to investigate the genetic diversity in the genus “Candidatus Phytoplasma” is underlined.IMPORTANCE Coconut is an important crop for both industry and small stakeholders in many intertropical countries. Phytoplasma-associated lethal yellowing-like diseases have become one of the major pests that limit coconut cultivation as they have emerged in different parts of the world. We developed a multilocus sequence typing scheme (MLST) for tracking epidemics of “Ca. Phytoplasma palmicola,” which is responsible for coconut lethal yellowing disease (CLYD) on the African continent. MLST analysis applied to diseased coconut samples collected in western and eastern African countries also showed the existence of three distinct populations of “Ca. Phytoplasma palmicola” with low intrapopulation diversity. The reasons for the observed strong geographic patterns remain to be established but could result from the lethality of CLYD and the dominance of short-distance insect-mediated transmission.
机译：为了维持对椰子致死性黄化病（CLYD）的流行病学研究，椰子致死性黄化病是由植物原质引起的非洲毁灭性疾病，我们基于八个管家基因，针对“棕榈原虫”开发了多基因座序列分型（MLST）方案。在大陆一级，在加纳，尼日利亚和莫桑比克收集的132个受“ Cyidatus Phytoplasma palmicola”感染的椰子中，鉴定出八种不同的序列类型，而CLYD流行仍很活跃。 “棕榈假单胞菌”似乎是一个受到严重瓶颈影响的细菌，减少了将积极选择的有益突变固定到细菌种群中的现象。这种现象以及有限的植物寄主范围，可能解释了观察到的八种单倍型在特定国家的分布。作为增加适应性的替代方法，细菌还可以进行基因交换。但是，没有发现“棕榈假单胞菌”发生这种重组事件的证据。讨论了对CLYD流行病学和预防控制的意义。强调了七个看家基因对调查“ Candidatus phytoplasma”属遗传多样性的有用性。重要提示椰子是许多热带国家的工业和小型利益相关者的重要作物。与植原体相关的致死性黄化病害已成为限制椰子种植的主要害虫之一，因为它们已在世界各地出现。我们开发了一种多位点序列分型方案（MLST），用于跟踪“ Ca. “ Phytoplasma palmicola”，是造成非洲大陆椰子致命性黄化病（CLYD）的原因。对在西部和东部非洲国家采集的患病椰子样品进行的MLST分析还表明，存在三个不同的“ Ca”种群。种群内棕榈多样性低的Phytoplasma palmicola。观测到的强地理模式的原因尚待确定，但可能是由于CLYD的致死性和短距离昆虫介导的传播优势所致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号