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首页> 外文期刊>Applied Microbiology >Coordinated Regulation of the Size and Number of Polyhydroxybutyrate Granules by Core and Accessory Phasins in the Facultative Microsymbiont Sinorhizobium fredii NGR234
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Coordinated Regulation of the Size and Number of Polyhydroxybutyrate Granules by Core and Accessory Phasins in the Facultative Microsymbiont Sinorhizobium fredii NGR234

机译：兼性微共生fredrhizobium fredii NGR234中的核心和辅助蛋白对多羟基丁酸酯颗粒的大小和数量的协调调节。

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The exact roles of various granule-associated proteins (GAPs) of polyhydroxybutyrate (PHB) are poorly investigated, particularly for bacteria associated with plants. In this study, four structural GAPs, named phasins PhaP1 to PhaP4, were identified and demonstrated as true phasins colocalized with PHB granules in Sinorhizobium fredii NGR234, a facultative microsymbiont of Vigna unguiculata and many other legumes. The conserved PhaP2 dominated in regulation of granule size under both free-living and symbiotic conditions. PhaP1, another conserved phasin, made a higher contribution than accessory phasins PhaP4 and PhaP3 to PHB biosynthesis at stationary phase. PhaP3, with limited phyletic distribution on the symbiosis plasmid of Sinorhizobium, was more important than PhaP1 in regulating PHB biosynthesis in V. unguiculata nodules. Under the test conditions, no significant symbiotic defects were observed for mutants lacking individual or multiple phaP genes. The mutant lacking two PHB synthases showed impaired symbiotic performance, while mutations in individual PHB synthases or a PHB depolymerase yielded no symbiotic defects. This phenomenon is not related to either the number or size of PHB granules in test mutants within nodules. Distinct metabolic profiles and cocktail pools of GAPs of different phaP mutants imply that core and accessory phasins can be differentially involved in regulating other cellular processes in the facultative microsymbiont S. fredii NGR234.IMPORTANCE Polyhydroxybutyrate (PHB) granules are a store of carbon and energy in bacteria and archaea and play an important role in stress adaptation. Recent studies have highlighted distinct roles of several granule-associated proteins (GAPs) in regulating the size, number, and localization of PHB granules in free-living bacteria, though our knowledge of the role of GAPs in bacteria associated with plants is still limited. Here we report distinct roles of core and accessory phasins associated with PHB granules of Sinorhizobium fredii NGR234, a broad-host-range microsymbiont of diverse legumes. Core phasins PhaP2 and PhaP1 are conserved major phasins in free-living cells. PhaP2 and accessory phasin PhaP3, encoded by an auxiliary gene on the symbiosis plasmid, are major phasins in nitrogen-fixing bacteroids in cowpea nodules. GAPs and metabolic profiles can vary in different phaP mutants. Contrasting symbiotic performances between mutants lacking PHB synthases, depolymerase, or phasins were revealed.
机译：聚羟基丁酸酯（PHB）的各种颗粒相关蛋白（GAP）的确切作用尚未得到充分研究，特别是对于与植物相关的细菌。在这项研究中，鉴定了四个结构性GAP，分别为Phsins PhaP1至PhaP4，并证明它们与PHB颗粒共定位于真正的Phasins fredii NGR234，Vigna unguiculata的兼性微共生体和许多其他豆类中。在自由生活和共生条件下，保守的PhaP2在颗粒大小的调节中占主导地位。 PhaP1，另一种保守的phasin，在固定相方面对PHB生物合成的贡献高于辅助phasins PhaP4和PhaP3。 PhaP3在中华根瘤菌的共生质粒上具有有限的系统分布，在调节结缕草结节中的PHB生物合成方面比PhaP1更重要。在测试条件下，对于缺少单个或多个phaP基因的突变体，未观察到明显的共生缺陷。缺少两个PHB合酶的突变体显示共生性能受损，而单个PHB合酶的突变或PHB解聚酶没有共生缺陷。这种现象与结节内测试突变体中PHB颗粒的数量或大小无关。不同phaP突变体的GAP的不同代谢谱和鸡尾酒库暗示核心和辅助phasins可以差异地参与兼性微共生菌S.fredii NGR234的其他细胞过程的调控。重要聚羟基丁酸酯（PHB）颗粒是碳和能量的储存细菌和古细菌在压力适应中起重要作用。最近的研究强调了几种颗粒相关蛋白（GAP）在调节自由生活细菌中PHB颗粒的大小，数量和定位方面的独特作用，尽管我们对GAP在与植物相关的细菌中的作用的了解仍然有限。在这里，我们报告与Shierhizobium fredii NGR234的PHB颗粒相关的核心和辅助阶段蛋白的独特作用，Sherrhizobium fredii NGR234是一种广泛的宿主范围的多种豆类微共生体。核心阶段素PhaP2和PhaP1是自由活动细胞中保守的主要阶段素。由共生质粒上的辅助基因编码的PhaP2和辅助阶段蛋白PhaP3是cow豆结节中固氮类细菌中的主要阶段蛋白。 GAP和代谢谱在不同的phaP突变体中可能有所不同。揭示了缺乏PHB合酶，解聚酶或phasins的突变体之间相反的共生性能。

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