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Molecular Basis of the Behavior of Hepatitis A Virus Exposed to High Hydrostatic Pressure

机译：高静水压力下甲型肝炎病毒行为的分子基础

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Food-borne hepatitis A outbreaks may be prevented by subjecting foods at risk of virus contamination to moderate treatments of high hydrostatic pressure (HHP). A pretreatment promoting hepatitis A virus (HAV) capsid-folding changes enhances the virucidal effect of HHP, indicating that its efficacy depends on capsid conformation. HAV populations enriched in immature capsids (125S provirions) are more resistant to HHP, suggesting that mature capsids (150S virions) are more susceptible to this treatment. In addition, the monoclonal antibody (MAb) K24F2 epitope contained in the immunodominant site is a key factor for the resistance to HHP. Changes in capsid folding inducing a loss of recognition by MAb K24F2 render more susceptible conformations independently of the origin of such changes. Accordingly, codon usage-associated folding changes and changes stimulated by pH-dependent breathings, provided they confer a loss of recognition by MAb K24F2, induce a higher susceptibility to HHP. In conclusion, the resistance of HAV to HHP treatments may be explained by a low proportion of 150S particles combined with a good accessibility of the epitope contained in the immunodominant site close to the 5-fold axis.
机译：食源性甲型肝炎的暴发可以通过对有病毒污染风险的食品进行适度的高静水压（HHP）处理来预防。促进甲型肝炎病毒（HAV）衣壳折叠变化的预处理可增强HHP的杀病毒作用，表明其功效取决于衣壳构象。富含未成熟衣壳（125S病毒体）的HAV种群对HHP的抵抗力更高，表明成熟的衣壳（150S病毒体）对这种治疗更敏感。此外，免疫显性位点中包含的单克隆抗体（MAb）K24F2表位是抵抗HHP的关键因素。衣壳折叠的变化引起MAb K24F2识别能力的丧失，使得更易感的构象独立于这种变化的起源。因此，与密码子使用相关的折叠变化和受pH依赖的呼吸刺激的变化（前提是它们导致MAb K24F2的识别丧失）引起了对HHP的更高敏感性。总之，HAV对HHP治疗的抗性可以通过低比例的150S颗粒以及接近5倍轴的免疫优势位点中包含的表位的良好可达性来解释。

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