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首页> 外文期刊>Applied Microbiology >Cellulolytic Streptomyces Strains Associated with Herbivorous Insects Share a Phylogenetically Linked Capacity To Degrade Lignocellulose
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Cellulolytic Streptomyces Strains Associated with Herbivorous Insects Share a Phylogenetically Linked Capacity To Degrade Lignocellulose

机译：与草食性昆虫相关的纤维素分解链霉菌菌株在系统发育上具有降解木质纤维素的能力。

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Actinobacteria in the genus Streptomyces are critical players in microbial communities that decompose complex carbohydrates in the soil, and these bacteria have recently been implicated in the deconstruction of plant polysaccharides for some herbivorous insects. Despite the importance of Streptomyces to carbon cycling, the extent of their plant biomass-degrading ability remains largely unknown. In this study, we compared four strains of Streptomyces isolated from insect herbivores that attack pine trees: DpondAA-B6 (SDPB6) from the mountain pine beetle, SPB74 from the southern pine beetle, and SirexAA-E (SACTE) and SirexAA-G from the woodwasp, Sirex noctilio . Biochemical analysis of secreted enzymes demonstrated that only two of these strains, SACTE and SDPB6, were efficient at degrading plant biomass. Genomic analyses indicated that SACTE and SDPB6 are closely related and that they share similar compositions of carbohydrate-active enzymes. Genome-wide proteomic and transcriptomic analyses revealed that the major exocellulases (GH6 and GH48), lytic polysaccharide monooxygenases (AA10), and mannanases (GH5) were conserved and secreted by both organisms, while the secreted endocellulases (GH5 and GH9 versus GH9 and GH12) were from diverged enzyme families. Together, these data identify two phylogenetically related insect-associated Streptomyces strains with high biomass-degrading activity and characterize key enzymatic similarities and differences used by these organisms to deconstruct plant biomass.
机译：链霉菌属中的放线菌是分解土壤中复杂碳水化合物的微生物群落的关键角色，最近，这些细菌与某些草食性昆虫的植物多糖的分解有关。尽管链霉菌对碳循环很重要，但其植物生物质降解能力的程度仍是未知之数。在这项研究中，我们比较了从攻击松树的食草动物中分离出的四种链霉菌菌株：山松甲虫的DpondAA-B6（SDPB6），南松甲虫的SPB74和SirexAA-E（SACTE）和SirexAA-G木蜂，Sirex noctilio。分泌酶的生化分析表明，其中只有两个菌株SACTE和SDPB6可有效降解植物生物量。基因组分析表明，SACTE和SDPB6密切相关，并且它们具有类似的碳水化合物活性酶组成。全基因组蛋白质组学和转录组学分析表明，两种生物均能保护和分泌主要外切纤维素酶（GH6和GH48），裂解多糖单加氧酶（AA10）和甘露聚糖酶（GH5），而分泌的内切纤维素酶（GH5和GH9与GH9和GH12相比） ）来自不同的酶家族。在一起，这些数据确定了具有高生物量降解活性的两个系统发育相关的昆虫相关链霉菌菌株，并表征了这些生物用于解构植物生物量的关键酶相似性和差异性。

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