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首页> 外文期刊>Applied Microbiology >Inactivation of Murine Norovirus on a Range of Copper Alloy Surfaces Is Accompanied by Loss of Capsid Integrity
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Inactivation of Murine Norovirus on a Range of Copper Alloy Surfaces Is Accompanied by Loss of Capsid Integrity

机译：丧失衣壳完整性伴随着鼠诺如病毒在一系列铜合金表面上的失活

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Norovirus is one of the most common causes of acute viral gastroenteritis. The virus is spread via the fecal-oral route, most commonly from infected food and water, but several outbreaks have originated from contamination of surfaces with infectious virus. In this study, a close surrogate of human norovirus causing gastrointestinal disease in mice, murine norovirus type 1 (MNV-1), retained infectivity for more than 2 weeks following contact with a range of surface materials, including Teflon (polytetrafluoroethylene [PTFE]), polyvinyl chloride (PVC), ceramic tiles, glass, silicone rubber, and stainless steel. Persistence was slightly prolonged on ceramic surfaces. A previous study in our laboratory observed that dry copper and copper alloy surfaces rapidly inactivated MNV-1 and destroyed the viral genome. In this new study, we have observed that a relatively small change in the percentage of copper, between 70 and 80% in copper nickels and 60 and 70% in brasses, had a significant influence on the ability of the alloy to inactivate norovirus. Nickel alone did not affect virus, but zinc did have some antiviral effect, which was synergistic with copper and resulted in an increased efficacy of brasses with lower percentages of copper. Electron microscopy of purified MNV-1 that had been exposed to copper and stainless steel surfaces suggested that a massive breakdown of the viral capsid had occurred on copper. In addition, MNV-1 that had been exposed to copper and treated with RNase demonstrated a reduction in viral gene copy number. This suggests that capsid integrity is compromised upon contact with copper, allowing copper ion access to the viral genome.
机译：诺如病毒是急性病毒性胃肠炎的最常见原因之一。该病毒通过粪-口途径传播，最常见的是从被感染的食物和水传播，但是有几次爆发是由于感染性病毒对表面的污染所致。在这项研究中，人类诺如病毒引起小鼠胃肠道疾病的一种紧密替代品，鼠诺如病毒1型（MNV-1），在与包括聚四氟乙烯（聚四氟乙烯[PTFE]）在内的一系列表面材料接触后，仍保持了2周以上的传染性。 ，聚氯乙烯（PVC），瓷砖，玻璃，硅橡胶和不锈钢。在陶瓷表面的持久性略有延长。我们实验室先前的一项研究发现，干燥的铜和铜合金表面会迅速使MNV-1失活并破坏病毒基因组。在这项新研究中，我们观察到铜百分比的相对较小的变化（对铜镍而言为70％至80％，对于黄铜为60％至70％），对合金灭活诺如病毒的能力具有重大影响。单独的镍不会影响病毒，但是锌确实具有一定的抗病毒作用，与铜具有协同作用，并导致铜含量较低的黄铜的功效增强。暴露于铜和不锈钢表面的纯化MNV-1的电子显微镜观察表明，病毒衣壳在铜上发生了大规模分解。此外，已暴露于铜并用RNase处理的MNV-1表现出病毒基因拷贝数减少。这表明衣壳的完整性在与铜接触时受到损害，从而允许铜离子进入病毒基因组。

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