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首页> 外文期刊>Applied Microbiology >A Regulatory Feedback Loop between RpoS and SpoT Supports the Survival of Legionella pneumophila in Water
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A Regulatory Feedback Loop between RpoS and SpoT Supports the Survival of Legionella pneumophila in Water

机译：RpoS和SpoT之间的监管反馈回路支持退伍军人病菌在水中的生存

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摘要

Legionella pneumophila is a waterborne pathogen, and survival in the aquatic environment is central to its transmission to humans. Therefore, identifying genes required for its survival in water could help prevent Legionnaires' disease outbreaks. In the present study, we investigate the role of the sigma factor RpoS in promoting survival in water, where L. pneumophila experiences severe nutrient deprivation. The rpoS mutant showed a strong survival defect compared to the wild-type strain in defined water medium. The transcriptome of the rpoS mutant during exposure to water revealed that RpoS represses genes associated with replication, translation, and transcription, suggesting that the mutant fails to shut down major metabolic programs. In addition, the rpoS mutant is transcriptionally more active than the wild-type strain after water exposure. This could be explained by a misregulation of the stringent response in the rpoS mutant. Indeed, the rpoS mutant shows an increased expression of spoT and a corresponding decrease in the level of (p)ppGpp, which is due to the presence of a negative feedback loop between RpoS and SpoT. Therefore, the lack of RpoS causes an aberrant regulation of the stringent response, which prevents the induction of a successful response to starvation.
机译：嗜肺军团菌是一种水传播的病原体，在水生环境中的生存对其传播给人类至关重要。因此，鉴定其在水中生存所需的基因可以帮助预防退伍军人病的爆发。在本研究中，我们调查了西格玛因子RpoS在促进水中生存的作用，其中嗜肺乳杆菌经历了严重的营养剥夺。与野生型菌株在确定的水介质中相比，rpoS突变体显示出强大的生存缺陷。暴露于水中的rpoS突变体的转录组显示，RpoS抑制与复制，翻译和转录相关的基因，这表明该突变体无法关闭主要的代谢程序。此外，rpoS突变体在暴露于水后比野生型菌株在转录上更具活性。这可以通过rpoS突变体中严格应答的错误调节来解释。实际上，rpoS突变体显示spoT表达增加，（p）ppGpp水平相应降低，这是由于RpoS和SpoT之间存在负反馈回路所致。因此，缺乏RpoS会导致严格反应的异常调节，从而阻止诱导对饥饿的成功反应。

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