• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Bartonella chomelii Is the Most Frequent Species Infecting Cattle Grazing in Communal Mountain Pastures in Spain
【24h】

Bartonella chomelii Is the Most Frequent Species Infecting Cattle Grazing in Communal Mountain Pastures in Spain

机译：Barmelella chomelii是西班牙公共山牧场上最常见的牛感染物种

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The presence of Bartonella spp. was investigated in domestic ungulates grazing in communal pastures from a mountain area in northern Spain, where 18.3% (17/93) of cattle were found to be positive by PCR combined with a reverse line blot (PCR/RLB), whereas sheep ( n = 133) or horses ( n = 91) were found not to be infected by this pathogen. Bartonella infection was significantly associated with age, since older animals showed a higher prevalence than heifers and calves. In contrast to other studies, B. chomelii was the most frequent species found in cattle (14/17), while B. bovis was detected in only three animals. Moreover, 18 B. chomelii isolates and one B. bovis isolate were obtained from nine animals. Afterwards, B. chomelii isolates were characterized by a multilocus sequence typing (MLST) method which was adapted in this study. This method presented a high discrimination power, identifying nine different sequence types (STs). This characterization also showed the presence of different STs simultaneously in the same host and that STs had switched over time in one of the animals. In addition, B. chomelii STs seem to group phylogenetically in two different lineages. The only B. bovis isolate was characterized with a previously described MLST method. This isolate corresponded to a new ST which is located in lineage I, where the B. bovis strains infecting Bos taurus subsp. taurus are grouped. Further studies on the dynamics of Bartonella infection in cattle and the potential ectoparasites involved in the transmission of this microorganism should be performed, improving knowledge about the interaction of Bartonella spp. and domestic ungulates.
机译：巴尔通体属的存在。在西班牙北部山区的公共牧场放牧的有蹄类动物中进行了调查，通过PCR和反向线印迹（PCR / RLB）结合，发现18.3％（17/93）的牛为阳性，而绵羊（n = 133）或马（n = 91）未被该病原体感染。由于年龄较大的动物的患病率高于小母牛和犊牛，因此巴尔通体感染与年龄显着相关。与其他研究相反，霍乱双歧杆菌是在牛中发现的最常见物种（14/17），而仅在三只动物中发现了牛双歧杆菌。此外，从九只动物中获得了18种霍乱双歧杆菌和一种牛双歧杆菌。之后，通过多基因座序列分型（MLST）方法对霍乱双歧杆菌分离株进行了表征，该方法适用于本研究。该方法具有很高的辨别力，可识别九种不同的序列类型（ST）。此特征还表明在同一宿主中同时存在不同的ST，并且其中一只动物的ST随时间变化。另外，霍乱双歧杆菌ST似乎在两个不同的谱系上系统发育上分组。用先前描述的MLST方法表征唯一的牛双歧杆菌。该分离物对应于位于谱系I中的新的ST，其中感染了Bos taurus亚种的牛双歧杆菌菌株。金牛座被分组。应进一步研究牛中Bartonella感染的动力学以及涉及该微生物传播的潜在外寄生虫，以提高对Bartonella spp相互作用的认识。和有蹄类动物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号