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首页> 外文期刊>Applied Microbiology >Influence of Salmonella enterica Serovar Typhimurium ssrB on Colonization of Eastern Oysters (Crassostrea virginica) as Revealed by a Promoter Probe Screen
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Influence of Salmonella enterica Serovar Typhimurium ssrB on Colonization of Eastern Oysters (Crassostrea virginica) as Revealed by a Promoter Probe Screen

机译：启动子探针筛选显示沙门氏菌血清型鼠伤寒沙门氏菌对东部牡蛎（Crassostrea virginica）定殖的影响

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Although Salmonella has been isolated from 7.4 to 8.6% of domestic raw oysters, representing a significant risk for food-borne illness, little is known about the factors that influence their initial colonization by Salmonella . This study tested the hypothesis that specific regulatory changes enable a portion of the invading Salmonella population to colonize oysters. An in vivo promoter probe library screen identified 19 unique regions as regulated during colonization. The mutants in the nearest corresponding downstream genes were tested for colonization defects in oysters. Only one mutation, in ssrB , resulted in a significantly reduced ability to colonize oysters compared to that of wild-type Salmonella . Because ssrB regulates Salmonella pathogenicity island 2 (SPI-2)-dependent infections in vertebrate macrophages, the possibility that ssrB mediated colonization of oyster hemocytes in a similar manner was examined. However, no difference in hemocyte colonization was observed. The complementary hypothesis that signal exchange between Salmonella and the oyster's native microbial community aids colonization was also tested. Signals that triggered responses in quorum sensing (QS) reporters were shown to be produced by oyster-associated bacteria and present in oyster tissue. However, no evidence for signal exchange was observed in vivo . The sdiA reporter responded to salinity, suggesting that SdiA may also have a role in environmental sensing. Overall, this study suggests the initial colonization of live oysters by Salmonella is controlled by a limited number of regulators, including ssrB .
机译：尽管沙门氏菌已被分离出占国内生牡蛎的7.4％至8.6％，这代表着食源性疾病的重大风险，但对于影响沙门氏菌最初定殖的因素知之甚少。这项研究检验了以下假设，即特定的法规变化使一部分入侵沙门氏菌种群能够定居于牡蛎。体内启动子探针库筛选确定了在定殖过程中受调控的19个独特区域。测试了最近的相应下游基因中的突变体在牡蛎中的定居缺陷。与野生型沙门氏菌相比，ssrB中只有一个突变导致定居牡蛎的能力大大降低。因为ssrB调节脊椎动物巨噬细胞中沙门氏菌致病岛2（SPI-2）依赖的感染，所以检查了ssrB介导的牡蛎血细胞定居的可能性。然而，未观察到血细胞定植的差异。还检验了沙门氏菌和牡蛎的原生微生物群落之间信号交换有助于定居的补充假设。已显示在群体感应（QS）报告基因中触发响应的信号由与牡蛎相关的细菌产生，并存在于牡蛎组织中。但是，在体内没有观察到信号交换的证据。 sdiA记者对盐度做出了回应，表明SdiA可能在环境感知中也有作用。总的来说，这项研究表明沙门氏菌对活牡蛎的最初定殖受包括ssrB在内的有限调节剂的控制。

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