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首页> 外文期刊>Applied Microbiology >Alcaligenes faecalis ZD02, a Novel Nematicidal Bacterium with an Extracellular Serine Protease Virulence Factor
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Alcaligenes faecalis ZD02, a Novel Nematicidal Bacterium with an Extracellular Serine Protease Virulence Factor

机译：Alcaligenes faecalis ZD02，一种具有细胞外丝氨酸蛋白酶致病因子的新型杀线虫细菌

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Root knot nematodes (RKNs) are the world's most damaging plant-parasitic nematodes (PPNs), and they can infect almost all crops. At present, harmful chemical nematicides are applied to control RKNs. Using microbial nematicides has been proposed as a better management strategy than chemical control. In this study, we describe a novel nematicidal bacterium named Alcaligenes faecalis ZD02. A. faecalis ZD02 was isolated from Caenorhabditis elegans cadavers and has nematostatic and nematicidal activity, as confirmed by C. elegans growth assay and life span assay. In addition, A. faecalis ZD02 fermentation broth showed toxicity against C. elegans and Meloidogyne incognita . To identify the nematicidal virulence factor, the genome of strain ZD02 was sequenced. By comparing all of the predicted proteins of strain ZD02 to reported nematicidal virulence factors, we determined that an extracellular serine protease (Esp) has potential to be a nematicidal virulence factor, which was confirmed by bioassay on C. elegans and M. incognita . Using C. elegans as the target model, we found that both A. faecalis ZD02 and the virulence factor Esp can damage the intestines of C. elegans . The discovery that A. faecalis ZD02 has nematicidal activity provides a novel bacterial resource for the control of RKNs.
机译：根结线虫（RKN）是世界上最具破坏性的植物寄生线虫（PPN），它们可以感染几乎所有农作物。目前，有害的杀线虫剂被用于控制RKNs。已经提出使用杀微生物剂作为比化学控制更好的管理策略。在这项研究中，我们描述了一种新型的杀线虫细菌，称为粪便产臭杆菌ZD02。从秀丽隐杆线虫尸体中分离出粪曲霉ZD02，并具有抑线虫和杀线虫活性，这通过秀丽隐杆线虫生长测定法和寿命测定法得以证实。此外，粪肠球菌ZD02发酵液对秀丽隐杆线虫和南方根结线虫具有毒性。为了鉴定杀线虫毒力因子，对菌株ZD02的基因组进行了测序。通过将菌株ZD02的所有预测蛋白与已报道的杀线虫毒力因子进行比较，我们确定胞外丝氨酸蛋白酶（Esp）有潜力成为杀线虫毒力因子，这已通过线虫和隐孢子虫的生物测定得到证实。使用秀丽隐杆线虫作为目标模型，我们发现屎肠球菌ZD02和毒力因子Esp均可破坏秀丽隐杆线虫的肠。粪肠球菌ZD02具有杀线虫活性的发现为控制RKNs提供了新的细菌资源。

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