• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Arabinose Induces Pellicle Formation by Vibrio fischeri
【24h】

Arabinose Induces Pellicle Formation by Vibrio fischeri

机译：阿拉伯糖诱导费氏弧菌形成膜层

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Biofilms are multicellular communities of bacteria attached to a surface and embedded in a protective matrix. In many cases, the signals that induce biofilm formation are unknown. Here, we report that biofilm formation by the marine bacterium Vibrio fischeri can be induced by the addition of arabinose to LBS (Luria-Bertani-salt), a tryptone-based medium. Growth of cells in the presence of 0.2% arabinose, but not other sugars, induced the production of a pellicle at the air/liquid interfaces of static cultures. V. fischeri failed to grow on arabinose as the sole carbon source, suggesting that pellicle production did not occur as a result of increased growth, but experiments using the acid/base indicator phenol red suggested that V. fischeri may partially metabolize arabinose. Pellicle production was independent of the syp polysaccharide locus but was altered upon disruption of the bcs cellulose locus. Through a screen for mutants defective for pellicle production, we found that loss of motility disrupted the formation of the arabinose-induced pellicle. Among the ～20 mutants that retained motility were strains with insertions in a putative msh pilus locus and a strain with a defect in yidK , which is involved in galactose catabolism. Mutants with the msh gene disrupted grew poorly in the presence of arabinose, while the yidK mutant appeared to be “blind” to the presence of arabinose. Finally, arabinose impaired symbiotic colonization by V. fischeri . This work thus identifies a novel signal and new pathways involved in control of biofilm formation by V. fischeri .
机译：生物膜是附着在表面并嵌入保护性基质中的细菌的多细胞群落。在许多情况下，诱导生物膜形成的信号是未知的。在这里，我们报告说，通过向基于胰蛋白p的培养基LBS（Luria-Bertani-salt）中添加阿拉伯糖，可以诱导海洋细菌费氏弧菌形成生物膜。在存在0.2％阿拉伯糖而不是其他糖的情况下，细胞的生长诱导了静态培养物的气/液界面处的防护膜的产生。费氏弧菌不能在阿拉伯糖上作为唯一的碳源生长，这表明生长增加不会导致表膜的生产，但是使用酸/碱指示剂酚红的实验表明，费氏弧菌可能部分代谢了阿拉伯糖。膜细胞的产生与syp多糖基因座无关，但在破坏bcs纤维素基因座后发生改变。通过筛选膜组件产生缺陷的突变体的筛选，我们发现运动能力的丧失破坏了阿拉伯糖诱导的膜组件的形成。在〜20个保留活力的突变体中，有一个在推定的msh菌毛基因座中插入的菌株，还有一个与半乳糖分解代谢有关的yidK缺陷的菌株。在阿拉伯糖的存在下，msh基因被破坏的突变体生长较差，而yidK突变体似乎对阿拉伯糖的存在是“盲目的”。最后，阿拉伯糖损害了费氏弧菌的共生定植。因此，这项工作确定了费氏弧菌控制生物膜形成的新信号和新途径。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号