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Finding targets for genome reduction in Streptomyces lividans TK24 using flux balance analysis

机译：使用通量平衡分析寻找 lividans链霉菌 TK24中的基因组还原目标

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Abstract: A genome reduction can be a good starting point for optimizing a wild-type strain for use in an industrial process. In a genome reduction, a selection of genes is removed with the intention of obtaining a robust strain with enhanced production properties. The complex soil bacterium Streptomyces lividans TK24 produces many compounds that can hinder the industrial objective. Genome reduction is often based on phylogenetic conservation and absolute gene expression. Here, flux balance analysis is proposed as an additional and complementary method for identifying potential targets for genome reduction. The effect of deletions of single metabolic genes and selected groups of adjacent metabolic genes on bacterial growth is simulated in a high throughput fashion. Targets identified through this method show lower gene expression and are less likely to be conserved between different Streptomyces strains. Gene expression data are used for assessing the effect of gene deletions in silico to improve prediction accuracy.
机译：摘要：基因组减少可能是优化用于工业过程的野生型菌株的良好起点。在基因组减少中，去除基因的选择是为了获得具有增强的生产特性的健壮菌株。复杂的土壤细菌lividans TK24会产生许多可能阻碍工业目标的化合物。基因组减少通常基于系统发育保守性和绝对基因表达。在这里，通量平衡分析被提议为一种额外的和补充的方法，用于识别基因组减少的潜在目标。以高通量方式模拟单个代谢基因和所选相邻代谢基因组的缺失对细菌生长的影响。通过这种方法鉴定的靶标显示较低的基因表达，并且在不同链霉菌菌株之间保守的可能性较小。基因表达数据用于评估计算机中基因缺失的效果，以提高预测准确性。

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