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Fusion detection in time-lapse microscopy images : application to lipid droplets coalescence in plant seeds

机译:时移显微镜图像中的融合检测:在植物种子中脂滴聚结中的应用

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Abstract: Detecting fusion events between lipid droplets of Arabidopsis thaliana embryos is of significant interest to understand the role of lipid droplet proteins. Lipid droplet proteins, called oleosins, have been shown to influence the size of lipid droplets possibly by preventing coalescence. We propose to detect fusion events in timelapse microscopy images of several oleosin deficient A. thaliana embryos. To detect volume preserving fusion events in a dense environment, we propose a procedure based on particle tracking and statistical tests. Using synthetic data, we compare the performances of our method to heuristic decision rules adapted from tracking algorithms from the literature.
机译:摘要:检测拟南芥胚胎脂质滴之间的融合事件对于了解脂质滴蛋白的作用具有重要意义。脂质液滴蛋白(称为油质蛋白)已显示可能通过防止聚结而影响脂质液滴的大小。我们建议在几个油质蛋白缺陷拟南芥胚胎的延时显微镜图像中检测融合事件。为了在密集环境中检测体积保留的融合事件,我们提出了一种基于粒子跟踪和统计测试的程序。使用合成数据,我们将我们的方法的性能与根据文献中的跟踪算法改编的启发式决策规则进行比较。

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