Subcellular Localization of Salmonella enteritidis Endotoxin in Liver and Spleen of Mice and Rats

James T. Willerson; Robert L. Trelstad; Theodore Pincus; Stuart B. Levy; Sheldon M. Wolff

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Subcellular Localization of Salmonella enteritidis Endotoxin in Liver and Spleen of Mice and Rats

机译：肠炎沙门氏菌内毒素在小鼠和大鼠肝脏和脾脏中的亚细胞定位

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Salmonella enteritidis14C-endotoxin was recovered predominantly from the nuclear and mitochondrial subcellular fractions of livers and spleens of mice and rats, 3.5 hr and 3 days after intravenous administration. Of the recovered radioactivity, 10 to 20% was present in the liver mitochondrial fraction as high-molecular-weight, biologically active material, suggesting the presence of intact endotoxin. Autoradiographic studies demonstrated nuclear and cytoplasmic labeling in the liver and at least nuclear label in spleen cells. The resistance of rats, as compared to mice, to the induction of amyloidosis does not appear to be based on a difference in subcellular localization of endotoxin within the reticuloendothelial system.

机译：肠内沙门氏菌 14 C-内毒素主要在静脉注射后3.5小时和3天从小鼠和大鼠的肝脏和脾脏的核和线粒体亚细胞部分回收。在回收的放射性中，肝线粒体部分中有10％至20％作为高分子量生物活性物质存在，表明存在完整的内毒素。放射自显影研究表明，肝脏中的核和细胞质标记以及脾细胞中的至少核标记。与小鼠相比，大鼠对淀粉样变性的诱导抗性似乎不是基于网状内皮系统内毒素亚细胞定位的差异。

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《Infection and immunity》 |1970年第5期|共6页
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James T. Willerson; Robert L. Trelstad; Theodore Pincus; Stuart B. Levy; Sheldon M. Wolff;
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Subcellular Localization of Salmonella enteritidis Endotoxin in Liver and Spleen of Mice and Rats

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