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Utilization of Amino Acids During Growth of Mycobacterium tuberculosis in Rotary Cultures

机译：旋转培养结核分枝杆菌生长过程中氨基酸的利用

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Marked differences were observed in the response of actively growing cells of the saprophyte, Mycobacterium smegmatis 607, and the avirulent human strain, M. tuberculosis (H37Ra), to several different nitrogen sources in aerated (rotary) cultures. The growth-promoting effect and utilization of equimolar concentrations (5 μmoles/ml) of l-alanine, l-aspartic acid, monosodium glutamate, or ammonium chloride were compared with that of l-asparagine, the normal nitrogen source, in Sauton synthetic liquid medium. The saprophyte grew equally well with each nitrogen source. However, marked differences were seen with H37Ra. Based on the rate of growth and cell yield, the relative growth-promoting effect of the amino acids for H37Ra is: alanine ? glutamate > asparagine > aspartic. Utilization of alanine, glutamate, and aspartic correlated well with growth. In contrast, utilization of asparagine during early growth of H37Ra was severalfold greater than that of either alanine or glutamate. Extracellular amino acids accumulated during the metabolism of asparagine but not during the utilization of the other nitrogen sources. Balanced metabolism of asparagine does not take place during aerated growth of H37Ra in asparagine media. During the metabolism of l-asparagine by M. tuberculosis (H37Ra) in aerated liquid cultures, metabolic controls may be exerted which impede protein synthesis.
机译：在腐生菌耻垢分枝杆菌 607和无毒力人类株 M的活跃生长细胞的反应中观察到明显差异。气（旋转）培养中的几种不同氮源。在Sauton合成液中比较了l-丙氨酸，l-天冬氨酸，谷氨酸一钠或氯化铵等摩尔浓度（5μmol/ ml）与正常氮源l-天冬酰胺的促生长作用和利用率。中。腐生植物在每种氮源下均生长良好。但是，H37Ra表现出明显差异。根据生长速率和细胞产量，氨基酸对H37Ra的相对促生长作用是：丙氨酸？谷氨酸>天冬酰胺>天冬氨酸。丙氨酸，谷氨酸和天冬氨酸的利用与生长良好相关。相反，H37Ra早期生长期间的天冬酰胺利用率比丙氨酸或谷氨酸高出几倍。细胞外氨基酸在天冬酰胺的代谢过程中积累，但在其他氮源的利用过程中不积累。在天冬酰胺培养基中H37Ra的充气生长期间，天冬酰胺的平衡代谢不会发生。在 M代谢l-天冬酰胺的过程中。加气液体培养中的结核病（H37Ra）可能会发挥代谢控制作用，从而阻碍蛋白质的合成。

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