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Antimycobacterial Effect of Lysates Prepared from Immunologically Activated Macrophages

机译：从免疫激活的巨噬细胞制备的裂解物的抗分枝杆菌作用

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Lysates or heptane extracts of peritoneal (P) and alveolar (A) normal macrophages (N-M), immune macrophages (I-M), and immune-activated macrophages (IA-M) were examined for antimycobacterial activity by the agar-plate diffusion test. This test has been found suitable to reveal the antibacterial activity in 3-day incubated, but not in freshly prepared, lysates. Results showed that materials of IA-AM or I-AM and of IA-PM exerted antimycobacterial effects, whereas materials of N-PM, I-PM, and of N-AM were usually inactive. Antimycobacterial activity of lysates of AM was stronger than that of PM. The formation of antibacterial factors during an incubation of M lysates, the solubility of the factors in heptane, and various other characteristics suggested that the antimycobacterial effect was caused by the formation of toxic levels of non-esterified fatty acids. M lysates exerted equal activities against BCG, H37Ra, and H37Rv strains of tubercle bacilli. The presence of antimycobacterial activity in lysates prepared from IA-M of either BCG- or BCG-sensitized animals indicated that the potential to generate antimycobacterial activity is associated with the state of delayed hypersensitivity and the state of activation of M.
机译：通过琼脂平板扩散试验检查腹膜（P）和肺泡（A）正常巨噬细胞（N-M），免疫巨噬细胞（I-M）和免疫激活巨噬细胞（IA-M）的裂解物或庚烷提取物的抗分枝杆菌活性。已经发现该测试适合于揭示在3天的温育中的抗菌活性，但不适用于新鲜制备的裂解物中。结果表明，IA-AM或I-AM以及IA-PM的材料具有抗分枝杆菌作用，而N-PM，I-PM和N-AM的材料通常没有活性。 AM的裂解物的抗分枝杆菌活性强于PM。 M裂解物孵育过程中抗菌因子的形成，因子在庚烷中的溶解度以及其他各种特征表明，抗分枝杆菌作用是由有毒水平的非酯化脂肪酸形成而引起的。裂解物对结核杆菌的BCG，H 37 Ra和H 37 Rv菌株具有相同的活性。由BCG或BCG致敏动物的IA-M制备的裂解物中存在抗分枝杆菌活性，这表明产生抗分枝杆菌活性的潜力与迟发型超敏反应的状态和M的激活状态有关。

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