首页> 外文期刊>Infection and immunity >A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lymphogranuloma Venereum Organisms
【24h】

A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lymphogranuloma Venereum Organisms

机译:沙眼包涵结膜炎-淋巴肉芽肿性生物的免疫分型的简化方法

获取原文
           

摘要

Responding to the pressure of large numbers of trachoma-inclusion conjunctivitis (TRIC)-lymphogranuloma venereum (LGV) isolates from field studies requiring serotyping, we have developed a simplified, less-precise method that utilizes cell culture-grown organisms to produce mouse antisera which is tested agaist prototype TRIC-LGV antigens in the micro-immunofluorescence test. Cell cultures with as few as 5 to 15% of cells showing inclusions produced adequate antibody in mice 4 days after single injection. Knowledge of the reaction of prototype antisera with the antigens has allowed typing of most isolates tested from the pattern of cross-reaction of their antiserum.
机译:针对需要进行血清学分型的田间研究中大量沙眼包涵体结膜炎(TRIC)-淋巴肉芽肿性腺(LGV)分离株的压力,我们开发了一种简化的,不太精确的方法,该方法利用细胞培养的生物体产生小鼠抗血清,在微量免疫荧光测试中对龙舌兰TRIC-LGV抗原原型进行了测试。单次注射后4天,只有5至15%的细胞培养物显示出内含物,从而在小鼠中产生了足够的抗体。了解原型抗血清与抗原的反应,可以根据其抗血清的交叉反应模式对大多数分离株进行分型。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号