...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Tissue-Binding Properties of the Cholera Toxin
【24h】

Tissue-Binding Properties of the Cholera Toxin

机译：霍乱毒素的组织结合特性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

[125I]choleragen was employed to study further the tissue-binding properties of highly purified choleragen. It was observed that [125I]choleragen was bound when combined with mucosal homogenates from all regions of the gastrointestinal tract of adult guinea pigs. Gastric, duodenal, jejunal, and ileal mucosa appeared equally effective in toxin-binding capacity. Preparations of large intestinal mucosa could bind an exceptionally larger amount of toxin. The binding property of small intestinal homogenates could not be attributed to any particular fraction after differential centrifugation; rather, the toxin receptor appeared to be associated with several sizes of particles containing cell membrane components. Although binding to mammalian cells was easily demonstrable, no binding to several types of bacterial cells was observed. The toxin receptor was found to be a “universal component” of many mammalian cell membranes, since specific binding of the toxin to a variety of guinea pig tissues was clearly demonstrated. [125I]choleragen binding to all tissues, with the exception of those prepared from brain and large intestinal mucosa, could be inhibited by preincubation of the tissue homogenates with unlabeled choleragen but not with comparable concentrations of normal rabbit serum proteins. The determination of the specificity of [125I]choleragen binding to brain and large intestinal mucosal homogenates was hampered by the continual release of soluble receptor from the homogenates, both of which contained the highest concentration of cholera toxin receptor. The data support and extend observations that cholera toxin binding to tissue receptor(s) is a very specific reaction, and further indicate that binding may occur with a variety of tissues to different degrees.
机译：[ 125 I]霍乱素被用来进一步研究高纯度霍乱素的组织结合特性。观察到[ 125 I]霍乱素与成年豚鼠胃肠道所有区域的粘膜匀浆结合时会结合。胃，十二指肠，空肠和回肠粘膜在毒素结合能力方面同样有效。大肠粘膜的制剂可以结合异常大量的毒素。小肠匀浆的结合特性不能归因于差异离心后的任何特定部分。相反，毒素受体似乎与包含细胞膜成分的多种大小的颗粒有关。尽管很容易证明与哺乳动物细胞的结合，但是未观察到与几种细菌细胞的结合。发现毒素受体是许多哺乳动物细胞膜的“通用成分”，因为已清楚地证明了毒素与多种豚鼠组织的特异性结合。 [ 125 I]霍乱素与所有组织的结合，但脑和大肠粘膜制备的结合物除外，可通过将组织匀浆与未标记的霍乱素一起预孵育而与正常兔相当浓度的结合来抑制血清蛋白。 [ 125 I]霍乱素与脑和大肠粘膜匀浆结合的特异性的测定受到匀浆中持续释放可溶性受体的阻碍，两者均含有最高浓度的霍乱毒素受体。数据支持并扩展了霍乱毒素与组织受体的结合是一种非常特殊的反应的观察结果，并进一步表明与多种组织的结合可能发生不同程度的结合。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号